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Team:Johns Hopkins/Data
From 2011.igem.org
Data Page
Vitamin A Production
Vitamin C Production
Yeast Toolkit
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530004 STM1p] |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530006 BAP2u] |
Integrating vectors |
Favorite New Parts
Vitamin A Production
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530000 CrtYB] Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is a fusion, it is the full Phytoene Synthase enzyme spliced with a Lycopene B-Cyclase enzymatic domain. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530001 CrtE] Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530002 CrtI] Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Phytoene Desaturase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes the conversion of Phytoene to Lycopene.
Vitamin C Production
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530025 VTC2] Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of GDP-L-Galactose to L-Galactose-1-P.
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530026 GME] Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of L-Galactose-1-P to L-Galactose.
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530027 VTC4] Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of L-Galactose to L-Galactono-1,4-Lactone.
Promoters
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530003 KRE9p] Promoter used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530008 TDH3p] Promoter used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
Terminators
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530019 HHO1utr] Terminator is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530020 ARD1utr] Terminator is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
Vectors
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530032 pRSBB413 HIS3 CEN/ARS Yeast Shuttle Vector] Used for the propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and HIS3 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector.
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530039 pRSBB426 URA3 2Micron Episomal Yeast Shuttle Vector] Used for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and URA3 selectable marker for S. cerevisiae. Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.
Existing Parts Characterized
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_J63006 Gal1p]. This characterization is a work in progress. We will update the part's experience page after the regional jamboree. We are working with the British Columbia team on troubleshooting.
All Submitted Parts
