Team:Waterloo

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Revision as of 20:57, 30 August 2011 by Honpeter (Talk | contribs)


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.


Ribozyme Project

The Ribozyme Project is the primary project for the Waterloo 2011 iGEM Team. It consists of a self-excising intron sequence which will provide a novel way to make fusion proteins without a ligation scar. For more information, please click on the links below to view the project description, safety, and up to date notebook entries.

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.

Project Abstract

Introns, self-excising ribozymes, can become a useful tool to create in vivo protein fusions of BioBrick parts. To make this possible, intron sequences are used to flank non-protein parts embedded in coding sequences. An intron sequence with an embedded recombination site is capable of in vivo insertion of a compatible protein fusion part. As an example, a GFP-fusion was created with an intervening lox site that is removed from the final protein using the intron to form a fully functional GFP protein. In vivo protein fusions can be applied to a larger number of modular systems to make complicated expression systems, such as synthetic antibodies or plants capable of Cry-toxin domain shuffling.

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