Team:Washington/Protocols/Digestion

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General Digestion Protocol

10 uL DNA

5uL buffer ( #2 for most, check http://www.neb.com/nebecomm/DoubleDigestCalculator.asp)

0.5 uL BSA

1uL enzyme 1

1uL enzyme 2

water to 50 uL (32.5 uL, add first)

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