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Team:Washington/Protocols
From 2011.igem.org
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(→Make It: Diesel Production Protocols) |
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[https://2011.igem.org/Team:Washington/Protocols/Gib_Purif. Gibson Purification] | [https://2011.igem.org/Team:Washington/Protocols/Gib_Purif. Gibson Purification] | ||
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| + | [https://2011.igem.org/Team:Washington/Protocols/High_PCR High-Yield PCR (Full-Gene Assembly)] | ||
[https://2011.igem.org/Team:Washington/Protocols/Plas_DNA. Isolation of Plasmid DNA (miniprep)] | [https://2011.igem.org/Team:Washington/Protocols/Plas_DNA. Isolation of Plasmid DNA (miniprep)] | ||
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[https://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures] | [https://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures] | ||
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=Wiki Design= | =Wiki Design= | ||
[https://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools (Wiki Markup, WikiDust, etc.)] | [https://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools (Wiki Markup, WikiDust, etc.)] | ||
Revision as of 04:39, 14 September 2011
General Protocols
General Agarose Gel Electrophoresis
General Transformation Protocol
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of how Kunkel Mutagensis works]
Standard 1L Expression Purification
Make It: Diesel Production Protocols
Alkane Biosynthesis media and extraction
Break It: Gluten Destruction Protocols
Small Scale (50mL) Protein Expression and Purification
Make It: Magnetosome Protocols
Electroporation (Transformation)
High-Yield PCR (Full-Gene Assembly)
Isolation of Plasmid DNA (miniprep)
Preparation of Overnight Cultures
Wiki Design
Wiki Design Tools (Wiki Markup, WikiDust, etc.)
