"
Team:Washington/Protocols
From 2011.igem.org
(Difference between revisions)
| (5 intermediate revisions not shown) | |||
| Line 3: | Line 3: | ||
<center><big><big><big><big>'''Protocols'''</big></big></big></big></center><br><br> | <center><big><big><big><big>'''Protocols'''</big></big></big></big></center><br><br> | ||
| + | |||
| + | =General Protocols= | ||
[https://2011.igem.org/Team:Washington/Protocols/gel_electrophoresis General Agarose Gel Electrophoresis] | [https://2011.igem.org/Team:Washington/Protocols/gel_electrophoresis General Agarose Gel Electrophoresis] | ||
| Line 18: | Line 20: | ||
[https://2011.igem.org/Team:Washington/Protocols/Competent Competent Cell Prep Protocol] | [https://2011.igem.org/Team:Washington/Protocols/Competent Competent Cell Prep Protocol] | ||
| - | [https://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel | + | [https://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel Mutagenesis] |
| - | [http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of | + | [http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process] |
[https://2011.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification] | [https://2011.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification] | ||
| Line 51: | Line 53: | ||
| - | =Make It: | + | =Make It: iGEM Toolkits= |
[https://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol] | [https://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol] | ||
Latest revision as of 02:10, 29 September 2011
General Protocols
General Agarose Gel Electrophoresis
General Transformation Protocol
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]
Standard 1L Expression Purification
Make It: Diesel Production Protocols
Alkane Biosynthesis media and extraction
Cell Lysate Assay by Decarbonylase Redesign Team
Break It: Gluten Destruction Protocols
Small Scale (50mL) Protein Expression and Purification
Make It: iGEM Toolkits
Electroporation (Transformation)
High-Yield PCR (Full-Gene Assembly)
Isolation of Plasmid DNA (miniprep)
Induction Studies of Proteins Fusions (mam-sfGFP)
Preparation of Overnight Cultures
Wiki Design
Wiki Design Tools (Wiki Markup, WikiDust, etc.)
