Team:Washington/Protocols

From 2011.igem.org

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__NOTOC__
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<center><big><big><big><big>UW iGEM 2011: Protocols</big></big></big></big></center><br><br>
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<center><big><big><big><big>'''Protocols'''</big></big></big></big></center><br><br>
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=General Protocols=
[http://2011.igem.org/Team:Washington/Protocols/gel_electrophoresis  General Agarose Gel Electrophoresis]
[http://2011.igem.org/Team:Washington/Protocols/gel_electrophoresis  General Agarose Gel Electrophoresis]
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[http://2011.igem.org/Team:Washington/Protocols/Competent  Competent Cell Prep Protocol]
[http://2011.igem.org/Team:Washington/Protocols/Competent  Competent Cell Prep Protocol]
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[http://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel Mutagensis]
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[http://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel Mutagenesis]
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[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of how Kunkel Mutagensis works]
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[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]
[http://2011.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification]
[http://2011.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification]
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[http://2011.igem.org/Team:Washington/Protocols/50mL_Scale Small Scale (50mL) Protein Expression and Purification]
[http://2011.igem.org/Team:Washington/Protocols/50mL_Scale Small Scale (50mL) Protein Expression and Purification]
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[http://2011.igem.org/Team:Washington/Protocols/Purified_Enzyme_Assay Purified Enzyme Assay]
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[http://2011.igem.org/Team:Washington/Protocols/Purified_Enzyme_Assay Enzyme Assay]
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=Make It: iGem Toolkits=
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=Make It: iGEM Toolkits=
[http://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol]
[http://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol]
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[http://2011.igem.org/Team:Washington/Protocols/Induc_studies. Induction Studies of Proteins Fusions (mam-sfGFP)]
[http://2011.igem.org/Team:Washington/Protocols/Induc_studies. Induction Studies of Proteins Fusions (mam-sfGFP)]
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[http://2011.igem.org/Team:Washington/Protocols/pGA. pGA Vector Assay]
[http://2011.igem.org/Team:Washington/Protocols/PBS. PBS Stock Protocol]
[http://2011.igem.org/Team:Washington/Protocols/PBS. PBS Stock Protocol]
[http://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures]
[http://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures]
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=Wiki Design=
=Wiki Design=
[http://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools (Wiki Markup, WikiDust, etc.)]
[http://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools (Wiki Markup, WikiDust, etc.)]

Latest revision as of 02:10, 29 September 2011


Protocols


General Protocols

General Agarose Gel Electrophoresis

General PCR Protocol

General Digestion Protocol

General Ligation Protocol

General Transformation Protocol

Colony PCR Protocol

Competent Cell Prep Protocol

Kunkel Mutagenesis

Overview of the Kunkel Mutagenesis process

Standard 1L Expression Purification

Gene Assembly With Oligos

Sequencing

Computational Protein Design

Glycerol Stocks


Make It: Diesel Production Protocols

Alkane Biosynthesis media and extraction

Alkane Biosynthesis cloning

Cell Lysate Assay by Decarbonylase Redesign Team


Break It: Gluten Destruction Protocols

Whole Cell Lysate Assay

Small Scale (50mL) Protein Expression and Purification

Enzyme Assay


Make It: iGEM Toolkits

Cytometry Protocol

Electroporation (Transformation)

Gibson Cloning/Assembly

Gibson Purification

High-Yield PCR (Full-Gene Assembly)

Isolation of Plasmid DNA (miniprep)

Induction Studies of Proteins Fusions (mam-sfGFP)

pGA Vector Assay

PBS Stock Protocol

Preparation of Overnight Cultures


Wiki Design

Wiki Design Tools (Wiki Markup, WikiDust, etc.)