Team:UNIPV-Pavia/parts/characterized2.html

From 2011.igem.org

(Difference between revisions)
Line 33: Line 33:
<li>RBS30-AiiA-TT (E9) -> pTet-RBS30-AiiA-TT (E24)</li>
<li>RBS30-AiiA-TT (E9) -> pTet-RBS30-AiiA-TT (E24)</li>
<li>RBS31-AiiA-TT (E10, <partinfo>BBa_I13914</partinfo>, DNA planning) -> pTet-RBS31-AiiA-TT (E25)</li>
<li>RBS31-AiiA-TT (E10, <partinfo>BBa_I13914</partinfo>, DNA planning) -> pTet-RBS31-AiiA-TT (E25)</li>
-
<li>RBS32-AiiA-TT (E11, <partinfo>BBa_I13912</partinfo>, DNA planning) -> pTet-RBS33-AiiA-TT (E26, rebuilt existing part <partinfo>BBa_ J22071</partinfo>, 2008 only, Bad sequencing)</li>
+
<li>RBS32-AiiA-TT (E11, <partinfo>BBa_I13912</partinfo>, DNA planning) -> pTet-RBS32-AiiA-TT (E26, rebuilt existing part <partinfo>BBa_ J22071</partinfo>, 2008 only, Bad sequencing)</li>
<li>RBS34-AiiA-TT (<partinfo>BBa_I0460</partinfo>) -> pTet-RBS34-AiiA-TT (E27)</li>
<li>RBS34-AiiA-TT (<partinfo>BBa_I0460</partinfo>) -> pTet-RBS34-AiiA-TT (E27)</li>
</ul>
</ul>

Revision as of 21:46, 7 September 2011

UNIPV TEAM 2011

Our new parts

BBa_J23101

  • BBa_J23101-RBS30-mRFP-TT (J101-E5)
  • BBa_J23101-RBS31-mRFP-TT (J101-31)
  • BBa_J23101-RBS32-mRFP-TT (J101-E7)

Genetic controller

Characterization of existing pars from the Registry

LuxI - BBa_C0061

  • RBS30-LuxI (E2 - Rebuilt existing part BBa_K081008, Registry 2011) -> pTet-RBS30-LuxI (E13)
  • RBS31-LuxI (E3) -> pTet-RBS31-LuxI (E14)
  • RBS32-LuxI (E4) -> pTet-RBS32-LuxI (E15)
  • RBS34-LuxI (BBa_C0261) -> pTet-RBS34-LuxI (E16, rebuilt existing part BBa_S03623, DNA available, only 2008 kit, inconsistent)

AiiA - BBa_C0060

  • RBS30-AiiA-TT (E9) -> pTet-RBS30-AiiA-TT (E24)
  • RBS31-AiiA-TT (E10, BBa_I13914, DNA planning) -> pTet-RBS31-AiiA-TT (E25)
  • RBS32-AiiA-TT (E11, BBa_I13912, DNA planning) -> pTet-RBS32-AiiA-TT (E26, rebuilt existing part BBa_ J22071, 2008 only, Bad sequencing)
  • RBS34-AiiA-TT (BBa_I0460) -> pTet-RBS34-AiiA-TT (E27)

pTet - BBa_R0040

  • pTet-RBS30-mRFP-TT (E21)
  • pTet-RBS31-mRFP-TT (E22)
  • pTet-RBS32-mRFP-TT (E23, Existing part BBa_I20252, DNA planning)
  • pTet-RBS34-mRFP-TT (BBa_I13521)
  • pTet-RBS30-LuxI (E13)
  • pTet-RBS31-LuxI (E14)
  • pTet-RBS32-LuxI (E15)
  • pTet-RBS34-LuxI (E16, rebuilt existing part BBa_S03623, DNA available, only 2008 kit, inconsistent)

RBSs BBa_B0030, BBa_B0031, BBa_B0032, BBa_B0034

  • RBS30-mRFP-TT(E5, rebuilt existing part from BBa_S04180, DNA planning) -> pTet-RBS30-mRFP-TT (E21)
  • RBS31-mRFP-TT(E6) -> pTet-RBS31-mRFP-TT (E22)
  • RBS32-mRFP-TT(E7, rebuilt existing part from BBa_ J133000, DNA planning) -> pTet-RBS32-mRFP-TT (E23, rebuilt existing part from BBa_I20252, DNA planning)
  • pTet-RBS34-mRFP-TT (BBa_I13521)
  • RBS30-LuxI (E2 - Rebuilt existing part BBa_K081008, Registry 2011) -> pTet-RBS30-LuxI (E13)
  • RBS31-LuxI (E3) -> pTet-RBS31-LuxI (E14)
  • RBS32-LuxI (E4) -> pTet-RBS32-LuxI (E15)
  • RBS34-LuxI (BBa_C0261) -> pTet-RBS34-LuxI (E16, rebuilt existing part BBa_S03623, DNA available, only 2008 kit, inconsistent)

Improvement of existing pars from the Registry

mRFP

  • RBS30-mRFP-TT (E5) -> pTet-RBS30-RFP-TT and pLux-RBS30-mRFP-TT (E21)
  • RBS31-mRFP-TT (E6) -> pTet-RBS31-RFP-TT and pLux-RBS31-mRFP-TT (E22)
  • RBS32-mRFP-TT (E7) -> pTet-RBS34-RFP-TT and pLux-RBS32-mRFP-TT (E23)
  • RBS34-mRFP-TT (BBa_I13507) -> pTet-RBS34-mRFP-TT BBa_I13521

pLux

  • Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT (E17)
  • Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT (E17)
  • Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT (E17)
  • Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT (E17)

Laboratory for Biomedical Informatics | Centre of Tissutal Engineering

University of Pavia
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