Team:UNIPV-Pavia/Freezer

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UNIPV TEAM 2011

FREEZER


For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look here.

Biobrick name Description Store Length Strain Vector Quality Control Creation Date
BBa_K081022 Plambda-RBS30-luxR-T(B1006)-pLux BioBricks 2011 969* E. coli TOP10 pSB1A2 Seq ok (MIT) 29/06/2011
BBa_C0060 (aiiA) autoinducer inactivation enzyme BioBricks 2011 789* E. coli TOP10 pSB1A2 Seq ok (MIT) 29/06/2011
BBa_B0015 (TT) double terminator 2009 129 E. coli TOP10 pSB1AK3 Seq ok (MIT) FREEZER 2009 Dist
BBa_R0040 (pTet) TetR repressible promoter 2009 54 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_C0061 (luxI) autoinducer synthetase for AHL BioBricks 2011 618* E. coli TOP10 pSB1A2 Seq ok (MIT) 30/06/2011
BBa_I13501 (mRFP-TT) Screening plasmid intermediate requested from the Registry 843* E. coli TOP10 pSB1AK3 Seq ok (MIT) FREEZER Requested 2011
BBa_B0030 RBS30 (eff=0.6) 2009 15 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0031 RBS31 (eff=0.07) derivative of BBa_0030 2009 14 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0032 RBS32 (eff=0.3) 2009 13 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0034 RBS34 (eff=1) 2009 12 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_I13521 (pTet-RBS34-RFP-TT) Ptet mRFP Constitutive on mRFP 2010 923* E. coli TOP10 pSB1A3 Seq ok (MIT) FREEZER 2010 Dist
BBa_C0261 (RBS34-luxI) AHL-making Enzyme, luxI (+RBS) BioBricks 2011 661* E. coli TOP10 pSB1A2 Seq ok (MIT) 07/07/2011
BBa_I13507 (RBS34-mRFP-TT) Screening plasmid intermediate 2009 861* E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
pSB4C5 Low copy BioBrick standard vector - chloramphenicol antibiotic resistance 2009 3221 E. coli TOP10 pSB4C5 Seq ok (MIT) FREEZER 2009 Dist
pSB3K3 Low to medium copy BioBrick standard vector - kanamycin resistance BioBricks 2011 2750 E. coli TOP10 pSB3K3 Seq ok (MIT) 30/06/2011
BBa_K3000005 GFP (Silver Standard Prefix) with terminator 2010 854 E. coli TOP10 pSB1C3 Seq ok (BMR Gemonics) FREEZER 2010 Ligations


Biobrick name Description Ligation Length Strain Vector Quality Control Creation Date
E1-2 aiiA-TT C0060(E-S)+B0015(E-X) 951* E. coli TOP10 pSB1AK3 Length OK 07/07/2011
E2-2 RBS30-LuxI C0061(X-P)+B0030(S-P) 664* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E3-1 RBS31-LuxI C0061(X-P)+B0031(S-P) 663* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E4-2 RBS32-LuxI C0061(X-P)+B0032(S-P) 662* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E5-2 RBS30-mRFP-TT I13501(X-P)+B0030(S-P) 864* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E6-1 RBS31-mRFP-TT I13501(X-P)+B0031(S-P) 863* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E7-2 RBS32-mRFP-TT I13501(X-P)+B0032(S-P) 862* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E8-3 Plambda-RBS30-luxR-T(B1006)-pLux K081022(E-P)+4C5(E-P) 969* E. coli TOP10 pSB4C5 Length OK 07/07/2011
E9-2 RBS30-aiiA-TT E1(X-P)+B0030(S-P) 972* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E10-1 RBS31-aiiA-TT E1(X-P)+B0031(S-P) 971* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E11-1 RBS32-aiiA-TT E1(X-P)+B0032(S-P) 970* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E12-1 RBS34-aiiA-TT E1(X-P)+ B0034(S-P) 969* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E13-1 pTet-RBS30-LuxI E2(X-P)+R0040(S-P) 726* E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E14 pTet-RBS31-LuxI E3(X-P)+R0040(S-P) 725* E. coli TOP10 pSB1A2 FAILED
E15 pTet-RBS32-LuxI E4(X-P)+R0040(S-P) 724* E. coli TOP10 pSB1A2 FAILED
E16-1 pTet-RBS34-LuxI C0261(X-P)+R0040(S-P) 723* E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E17-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT E5(X-P)+E8(S-P) 1841* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E18-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT E6(X-P)+E8(S-P) 1840* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E19-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT E7(X-P)+E8(S-P) 1839* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E20-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT I13507(X-P)+E8(S-P) 1838* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E21-1 pTet-RBS30-mRFP-TT E5(X-P)+R0040(S-P) 926* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E22-2 pTet-RBS31-mRFP-TT E6(X-P)+R0040(S-P) 925* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E23-1 pTet-RBS32-mRFP-TT E7(X-P)+R0040(S-P) 924* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
BBa_R0040 in BBa_J61002 pTet in BBa_J61002 plasmid BBa_R0040(S-P)+BBa_J23101(S-P) 923* E. coli TOP10 BBa_J61002 Length OK 15/07/2011
E24-2 pTet-RBS30-aiiA-TT E9(X-P)+R0040(S-P) 1034* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E25-1 pTet-RBS31-aiiA-TT E10(X-P)+R0040(S-P) 1033* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E26-2 pTet-RBS32-aiiA-TT E11(X-P)+R0040(S-P) 1032* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E27-2 pTet-RBS34-aiiA-TT E12(X-P)+R0040(S-P) 1031* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E28-1 pTet-RBS30-luxI in pSB4C5 E13(E-P)+pSB4C5(E-P) 726* E. coli MGZ1 pSB4C5 Bad Seq (BMR Genomics) 21/07/2011
E29 pTet-RBS31-luxI in pSB4C5 E14(E-P)+pSB4C5(E-P) 725* E. coli MGZ1 pSB4C5 FAILED
E30 pTet-RBS32-luxI in pSB4C5 E15(E-P)+pSB4C5(E-P) 724* E. coli MGZ1 pSB4C5 FAILED
E31-1 pTet-RBS34-luxI in pSB4C5 E16(E-P)+pSB4C5(E-P) 723* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E32-2 pTet-RBS30-mRFP-TT in pSB4C5 E21(E-P)+pSB4C5(E-P) 926* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E33-2 pTet-RBS31-mRFP-TT in pSB4C5 E22(E-P)+pSB4C5(E-P) 925* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E34-1 pTet-RBS32-mRFP-TT in pSB4C5 E23(E-P)+pSB4C5(E-P) 924* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E35-2 pTet-RBS34-mRFP-TT in pSB4C5 I13521(E-P)+pSB4C5(E-P) 923* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E36 pTet-J61002 in pSB4C5 pTet-J61002(E-P)+pSB4C5(E-P) 923* E. coli TOP10 pSB4C5 25/07/2011
E37-2 pTet-RBS30-aiiA-TT in pSB4C5 E24(E-P)+pSB4C5(E-P) 1034* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E38-1 pTet-RBS31-aiiA-TT in pSB4C5 E25(E-P)+pSB4C5(E-P) 1033* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E39-1 pTet-RBS32-aiiA-TT in pSB4C5 E26(E-P)+pSB4C5(E-P) 1032* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E40-2 pTet-RBS34-aiiA-TT in pSB4C5 E27(E-P)+pSB4C5(E-P) 1031* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E41N-1 pTet-RBS31-luxI in pSB4C5 E36(S-P)+E3(X-P) 725* E. coli MGZ1 pSB4C5 04/08/2011
E42-1 pTet-RBS32-luxI in pSB4C5 E36(S-P)+ E4(X-P) 724* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E43-3 pTet-RBS30-luxI in pSB4C5 E36(S-P)+ E2(X-P) 726* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 25/08/2011
E44-? pTet-RBS30-mRFP-TT BBa_R0040 in BBa_J61002 (S-P) + E5-2(X-P) 926* E. coli TOP10 BBa_J61002 ??? ???
E45-? pTet-RBS31-mRFP-TT BBa_R0040 in BBa_J61002 (S-P) + E6-1(X-P) 925* E. coli TOP10 BBa_J61002 ??? ???
E46-? pTet-RBS31-mRFP-TT BBa_R0040 in BBa_J61002 (S-P) + E7-2(X-P) 924* E. coli TOP10 BBa_J61002 ??? ???
E47-1 pTet-RBS34-mRFP-TT BBa_R0040 in BBa_J61002 (E-P) + pSB1C3 (E-P) 923* E. coli TOP10 pSB1C3 Length OK 04/09/2011
J101-E5 J23101-RBS30-mRFP-TT in pSB4C5 BBa_J23101(S-P)+ E5(S-P) 905* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
J101-31 J23101-RBS31-mRFP-TT in pSB4C5 BBa_J23101(S-P)+ E6(S-P) 904* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
J101-E7 J23101-RBS32-mRFP-TT in pSB4C5 BBa_J23101(S-P)+ E7(S-P) 903* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
J101-4C5 J23101-RBS34-mRFP-TT in pSB4C5 BBa_J23101(E-P)+ pSB4C5(E-P) 902* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
E3O-1C3-? RBS31-LuxI E3-1(E-P)+ pSB1C3 (E-P) 663* E. coli TOP10 pSB1C3 ??? 07/09/2011
E4N-1C3-? RBS32-LuxI E4-2(E-P)+ pSB1C3 (E-P) 662* E. coli TOP10 pSB1C3 ??? ???
E5-1C3 RBS30-mRFP-TT E5-2(E-P)+ pSB1C3 (E-P) 864* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E6-1C3 RBS31-mRFP-TT E6-1(E-P)+ pSB1C3 (E-P) 863* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E7-1C3 RBS32-mRFP-TT E7-2(E-P)+ pSB1C3 (E-P) 862* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E9-1C3 RBS30-aiiA-TT E9-2(E-P)+ pSB1C3 (E-P) 972* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E10-1C3 RBS31-aiiA-TT E10-1(E-P)+ pSB1C3 (E-P) 971* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
E11-1C3 RBS32-aiiA-TT E11-1(E-P)+ pSB1C3 (E-P) 970* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
J101-E5-1C3 J23101-RBS30-mRFP-TT J101-E5 (E-P) + pSB1C3(E-P) 905* E. coli TOP10 pSB1C3 Seq ok (BMR Genomics) 29/08/2011
J101-31-C3-1 J23101-RBS31-mRFP-TT J101-31 (E-P) + pSB1C3(E-P) 904* E. coli TOP10 pSB1C3 ??? 04/09/2011
J101-E7-1C3-1 J23101-RBS32-mRFP-TT J101-E7 (E-P) + pSB1C3(E-P) 903* E. coli TOP10 pSB1C3 Seq OK (BMR Gemonics) 29/08/2011

* The sequences of these parts may contain at least a 25 bp barcode.

Laboratory for Biomedical Informatics | Centre of Tissutal Engineering

University of Pavia
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