Team:UNIPV-Pavia/Freezer

From 2011.igem.org

(Difference between revisions)
Line 42: Line 42:
       <td class="row">(aiiA) autoinducer inactivation enzyme</td>
       <td class="row">(aiiA) autoinducer inactivation enzyme</td>
       <td class="row">BioBricks 2011</td>
       <td class="row">BioBricks 2011</td>
-
       <td class="row">789+25<sup>*</sup></td>
+
       <td class="row">789<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 78: Line 78:
       <td class="row">(luxI) autoinducer synthetase for AHL</td>
       <td class="row">(luxI) autoinducer synthetase for AHL</td>
       <td class="row">BioBricks 2011</td>
       <td class="row">BioBricks 2011</td>
-
       <td class="row">618+25<sup>*</sup></td>
+
       <td class="row">618<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 90: Line 90:
       <td class="row">(mRFP-TT) Screening plasmid intermediate</td>
       <td class="row">(mRFP-TT) Screening plasmid intermediate</td>
       <td class="row">requested from the Registry</td>
       <td class="row">requested from the Registry</td>
-
       <td class="row">843 (25 included)<sup>*</sup></td>
+
       <td class="row">843<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1AK3</td>
       <td class="row">pSB1AK3</td>
Line 100: Line 100:
   <tr>
   <tr>
       <td class="row"><a href="http://partsregistry.org/Part:BBa_B0030">BBa_B0030</a></td>
       <td class="row"><a href="http://partsregistry.org/Part:BBa_B0030">BBa_B0030</a></td>
-
       <td class="row">RBS30 (eff=0.6) (SCAR BEFORE: 8bp)</td>
+
       <td class="row">RBS30 (eff=0.6)</td>
       <td class="row">2009</td>
       <td class="row">2009</td>
       <td class="row">15</td>
       <td class="row">15</td>
Line 151: Line 151:
       <td class="row">(pTet-RBS34-RFP-TT) Ptet mRFP Constitutive on mRFP</td>
       <td class="row">(pTet-RBS34-RFP-TT) Ptet mRFP Constitutive on mRFP</td>
       <td class="row">2010</td>
       <td class="row">2010</td>
-
       <td class="row">923(25 included)<sup>*</sup></td>
+
       <td class="row">923<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A3</td>
       <td class="row">pSB1A3</td>
Line 164: Line 164:
       <td class="row">(RBS34-luxI) AHL-making Enzyme, luxI (+RBS)</td>
       <td class="row">(RBS34-luxI) AHL-making Enzyme, luxI (+RBS)</td>
       <td class="row">BioBricks 2011</td>
       <td class="row">BioBricks 2011</td>
-
       <td class="row">661 (25 included)<sup>*</sup></td>
+
       <td class="row">661<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 175: Line 175:
       <td class="row">(RBS34-mRFP-TT) Screening plasmid intermediate</td>
       <td class="row">(RBS34-mRFP-TT) Screening plasmid intermediate</td>
       <td class="row">2009</td>
       <td class="row">2009</td>
-
       <td class="row">861 (25 included)<sup>*</sup></td>
+
       <td class="row">861<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 238: Line 238:
       <td class="row">aiiA-TT</td>
       <td class="row">aiiA-TT</td>
       <td class="row">C0060(E-S)+B0015(E-X)</td>
       <td class="row">C0060(E-S)+B0015(E-X)</td>
-
       <td class="row">926+25<sup>*</sup></td>
+
       <td class="row">951<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1AK3</td>
       <td class="row">pSB1AK3</td>
Line 249: Line 249:
       <td class="row">RBS30-LuxI</td>
       <td class="row">RBS30-LuxI</td>
       <td class="row">C0061(X-P)+B0030(S-P)</td>
       <td class="row">C0061(X-P)+B0030(S-P)</td>
-
       <td class="row">639+25<sup>*</sup></td>
+
       <td class="row">664<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 260: Line 260:
       <td class="row">RBS31-LuxI</td>
       <td class="row">RBS31-LuxI</td>
       <td class="row">C0061(X-P)+B0031(S-P)</td>
       <td class="row">C0061(X-P)+B0031(S-P)</td>
-
       <td class="row">638+25<sup>*</sup></td>
+
       <td class="row">663<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 271: Line 271:
       <td class="row">RBS32-LuxI</td>
       <td class="row">RBS32-LuxI</td>
       <td class="row">C0061(X-P)+B0032(S-P)</td>
       <td class="row">C0061(X-P)+B0032(S-P)</td>
-
       <td class="row">637+25<sup>*</sup></td>
+
       <td class="row">662<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 282: Line 282:
       <td class="row">RBS30-mRFP-TT</td>
       <td class="row">RBS30-mRFP-TT</td>
       <td class="row">I13501(X-P)+B0030(S-P)</td>
       <td class="row">I13501(X-P)+B0030(S-P)</td>
-
       <td class="row">864(25 included)<sup>*</sup></td>
+
       <td class="row">864<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 293: Line 293:
       <td class="row">RBS31-mRFP-TT</td>
       <td class="row">RBS31-mRFP-TT</td>
       <td class="row">I13501(X-P)+B0031(S-P)</td>
       <td class="row">I13501(X-P)+B0031(S-P)</td>
-
       <td class="row">863(25 included)<sup>*</sup></td>
+
       <td class="row">863<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 304: Line 304:
       <td class="row">RBS32-mRFP-TT</td>
       <td class="row">RBS32-mRFP-TT</td>
       <td class="row">I13501(X-P)+B0032(S-P)</td>
       <td class="row">I13501(X-P)+B0032(S-P)</td>
-
       <td class="row">862(25 included)<sup>*</sup></td>
+
       <td class="row">862<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 315: Line 315:
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux</td>
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux</td>
       <td class="row">K081022(E-P)+4C5(E-P)</td>
       <td class="row">K081022(E-P)+4C5(E-P)</td>
-
       <td class="row">969(25 included)<sup>*</sup></td>
+
       <td class="row">969<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 326: Line 326:
       <td class="row">RBS30-aiiA-TT</td>
       <td class="row">RBS30-aiiA-TT</td>
       <td class="row">E1(X-P)+B0030(S-P)</td>
       <td class="row">E1(X-P)+B0030(S-P)</td>
-
       <td class="row">947+25<sup>*</sup></td>
+
       <td class="row">972<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 337: Line 337:
       <td class="row">RBS31-aiiA-TT</td>
       <td class="row">RBS31-aiiA-TT</td>
       <td class="row">E1(X-P)+B0031(S-P)</td>
       <td class="row">E1(X-P)+B0031(S-P)</td>
-
       <td class="row">946+25<sup>*</sup></td>
+
       <td class="row">971<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 348: Line 348:
       <td class="row">RBS32-aiiA-TT</td>
       <td class="row">RBS32-aiiA-TT</td>
       <td class="row">E1(X-P)+B0032(S-P)</td>
       <td class="row">E1(X-P)+B0032(S-P)</td>
-
       <td class="row">945+25<sup>*</sup></td>
+
       <td class="row">970<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 359: Line 359:
       <td class="row">RBS34-aiiA-TT</td>
       <td class="row">RBS34-aiiA-TT</td>
       <td class="row">E1(X-P)+ B0034(S-P)</td>
       <td class="row">E1(X-P)+ B0034(S-P)</td>
-
       <td class="row">946+25<sup>*</sup></td>
+
       <td class="row">969<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 370: Line 370:
       <td class="row">pTet-RBS30-LuxI</td>
       <td class="row">pTet-RBS30-LuxI</td>
       <td class="row">E2(X-P)+R0040(S-P)</td>
       <td class="row">E2(X-P)+R0040(S-P)</td>
-
       <td class="row">701+25<sup>*</sup></td>
+
       <td class="row">726<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 381: Line 381:
       <td class="row">pTet-RBS31-LuxI</td>
       <td class="row">pTet-RBS31-LuxI</td>
       <td class="row">E3(X-P)+R0040(S-P)</td>
       <td class="row">E3(X-P)+R0040(S-P)</td>
-
       <td class="row">700+25<sup>*</sup></td>
+
       <td class="row">725<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 392: Line 392:
       <td class="row">pTet-RBS32-LuxI</td>
       <td class="row">pTet-RBS32-LuxI</td>
       <td class="row">E4(X-P)+R0040(S-P)</td>
       <td class="row">E4(X-P)+R0040(S-P)</td>
-
       <td class="row">699+25<sup>*</sup></td>
+
       <td class="row">724<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 403: Line 403:
       <td class="row">pTet-RBS34-LuxI</td>
       <td class="row">pTet-RBS34-LuxI</td>
       <td class="row">C0261(X-P)+R0040(S-P)</td>
       <td class="row">C0261(X-P)+R0040(S-P)</td>
-
       <td class="row">698??+25 (già 723)<sup>*</sup></td>
+
       <td class="row">723<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 414: Line 414:
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT</td>
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT</td>
       <td class="row">E5(X-P)+E8(S-P)</td>
       <td class="row">E5(X-P)+E8(S-P)</td>
-
       <td class="row">1841(25 included)<sup>*</sup></td>
+
       <td class="row">1841<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
Line 425: Line 425:
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT</td>
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT</td>
       <td class="row">E6(X-P)+E8(S-P)</td>
       <td class="row">E6(X-P)+E8(S-P)</td>
-
       <td class="row">1840(25 included)<sup>*</sup></td>
+
       <td class="row">1840<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
Line 436: Line 436:
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT</td>
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT</td>
       <td class="row">E7(X-P)+E8(S-P)</td>
       <td class="row">E7(X-P)+E8(S-P)</td>
-
       <td class="row">1839(25 included)<sup>*</sup></td>
+
       <td class="row">1839<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
Line 447: Line 447:
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT</td>
       <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT</td>
       <td class="row">I13507(X-P)+E8(S-P)</td>
       <td class="row">I13507(X-P)+E8(S-P)</td>
-
       <td class="row">1838(25 included)<sup>*</sup></td>
+
       <td class="row">1838<sup>*</sup></td>
-
       <td class="row">E. coli TOP10</td>
+
       <td class="row">E. coli TOP10/ E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">Seq ok (BMR Genomics)</td>
       <td class="row">13/07/2011</td>
       <td class="row">13/07/2011</td>
-
  </tr>
 
-
 
-
  <tr>
 
-
      <td class="row">E17-2</td>
 
-
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT</td>
 
-
      <td class="row">E5(X-P)+E8(S-P)</td>
 
-
      <td class="row">1839<sup>*</sup></td>
 
-
      <td class="row">E. coli MGZ1</td>
 
-
      <td class="row">pSB4C5</td>
 
-
      <td class="row">Seq ok (BMR Genomics)</td>
 
-
      <td class="row">29/07/2011</td>
 
-
  </tr>
 
-
 
-
  <tr>
 
-
      <td class="row">E18-2</td>
 
-
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT</td>
 
-
      <td class="row">E6(X-P)+E8(S-P)</td>
 
-
      <td class="row">1840<sup>*</sup></td>
 
-
      <td class="row">E. coli MGZ1</td>
 
-
      <td class="row">pSB4C5</td>
 
-
      <td class="row">Seq ok (BMR Genomics)</td>
 
-
      <td class="row">29/07/2011</td>
 
-
  </tr>
 
-
 
-
  <tr>
 
-
      <td class="row">E19-2</td>
 
-
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT</td>
 
-
      <td class="row">E7(X-P)+E8(S-P)</td>
 
-
      <td class="row">1839<sup>*</sup></td>
 
-
      <td class="row">E. coli MGZ1</td>
 
-
      <td class="row">pSB4C5</td>
 
-
      <td class="row">Seq ok (BMR Genomics)</td>
 
-
      <td class="row">29/07/2011</td>
 
-
  </tr>
 
-
 
-
  <tr>
 
-
      <td class="row">E20-2</td>
 
-
      <td class="row">Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT</td>
 
-
      <td class="row">I13507(X-P)+E8(S-P)</td>
 
-
      <td class="row">1838<sup>*</sup></td>
 
-
      <td class="row">E. coli MGZ1</td>
 
-
      <td class="row">pSB4C5</td>
 
-
      <td class="row">Seq ok (BMR Genomics)</td>
 
-
      <td class="row">29/07/2011</td>
 
   </tr>
   </tr>
Line 502: Line 458:
       <td class="row">pTet-RBS30-mRFP-TT</td>
       <td class="row">pTet-RBS30-mRFP-TT</td>
       <td class="row">E5(X-P)+R0040(S-P)</td>
       <td class="row">E5(X-P)+R0040(S-P)</td>
-
       <td class="row">926(25 included)<sup>*</sup></td>
+
       <td class="row">926<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 513: Line 469:
       <td class="row">pTet-RBS31-mRFP-TT</td>
       <td class="row">pTet-RBS31-mRFP-TT</td>
       <td class="row">E6(X-P)+R0040(S-P)</td>
       <td class="row">E6(X-P)+R0040(S-P)</td>
-
       <td class="row">925(25 included)<sup>*</sup></td>
+
       <td class="row">925<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 524: Line 480:
       <td class="row">pTet-RBS32-mRFP-TT</td>
       <td class="row">pTet-RBS32-mRFP-TT</td>
       <td class="row">E7(X-P)+R0040(S-P)</td>
       <td class="row">E7(X-P)+R0040(S-P)</td>
-
       <td class="row">924(25 included)<sup>*</sup></td>
+
       <td class="row">924<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 535: Line 491:
       <td class="row">pTet-RBS30-aiiA-TT</td>
       <td class="row">pTet-RBS30-aiiA-TT</td>
       <td class="row">E9(X-P)+R0040(S-P)</td>
       <td class="row">E9(X-P)+R0040(S-P)</td>
-
       <td class="row">1009+25<sup>*</sup></td>
+
       <td class="row">1034<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 546: Line 502:
       <td class="row">pTet-RBS31-aiiA-TT</td>
       <td class="row">pTet-RBS31-aiiA-TT</td>
       <td class="row">E10(X-P)+R0040(S-P)</td>
       <td class="row">E10(X-P)+R0040(S-P)</td>
-
       <td class="row">1008+25<sup>*</sup></td>
+
       <td class="row">1033<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 557: Line 513:
       <td class="row">pTet-RBS32-aiiA-TT</td>
       <td class="row">pTet-RBS32-aiiA-TT</td>
       <td class="row">E11(X-P)+R0040(S-P)</td>
       <td class="row">E11(X-P)+R0040(S-P)</td>
-
       <td class="row">1007+25<sup>*</sup></td>
+
       <td class="row">1032<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 568: Line 524:
       <td class="row">pTet-RBS34-aiiA-TT</td>
       <td class="row">pTet-RBS34-aiiA-TT</td>
       <td class="row">E12(X-P)+R0040(S-P)</td>
       <td class="row">E12(X-P)+R0040(S-P)</td>
-
       <td class="row">1006+25<sup>*</sup></td>
+
       <td class="row">1031<sup>*</sup></td>
       <td class="row">E. coli TOP10</td>
       <td class="row">E. coli TOP10</td>
       <td class="row">pSB1A2</td>
       <td class="row">pSB1A2</td>
Line 579: Line 535:
       <td class="row">pTet-RBS30-luxI in pSB4C5</td>
       <td class="row">pTet-RBS30-luxI in pSB4C5</td>
       <td class="row">E13(E-P)+pSB4C5(E-P)</td>
       <td class="row">E13(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">701+25<sup>*</sup></td>
+
       <td class="row">726<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 590: Line 546:
       <td class="row">pTet-RBS31-luxI in pSB4C5</td>
       <td class="row">pTet-RBS31-luxI in pSB4C5</td>
       <td class="row">E14(E-P)+pSB4C5(E-P)</td>
       <td class="row">E14(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">700+25<sup>*</sup></td>
+
       <td class="row">725<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 601: Line 557:
       <td class="row">pTet-RBS32-luxI in pSB4C5</td>
       <td class="row">pTet-RBS32-luxI in pSB4C5</td>
       <td class="row">E15(E-P)+pSB4C5(E-P)</td>
       <td class="row">E15(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">699+25<sup>*</sup></td>
+
       <td class="row">724<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 612: Line 568:
       <td class="row">pTet-RBS34-luxI in pSB4C5</td>
       <td class="row">pTet-RBS34-luxI in pSB4C5</td>
       <td class="row">E16(E-P)+pSB4C5(E-P)</td>
       <td class="row">E16(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">698+25<sup>*</sup></td>
+
       <td class="row">723<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 623: Line 579:
       <td class="row">pTet-RBS30-mRFP-TT in pSB4C5</td>
       <td class="row">pTet-RBS30-mRFP-TT in pSB4C5</td>
       <td class="row">E21(E-P)+pSB4C5(E-P)</td>
       <td class="row">E21(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">926(25 included)<sup>*</sup></td>
+
       <td class="row">926<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 634: Line 590:
       <td class="row">pTet-RBS31-mRFP-TT in pSB4C5</td>
       <td class="row">pTet-RBS31-mRFP-TT in pSB4C5</td>
       <td class="row">E22(E-P)+pSB4C5(E-P)</td>
       <td class="row">E22(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">925(25 included)<sup>*</sup></td>
+
       <td class="row">925<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 645: Line 601:
       <td class="row">pTet-RBS32-mRFP-TT in pSB4C5</td>
       <td class="row">pTet-RBS32-mRFP-TT in pSB4C5</td>
       <td class="row">E23(E-P)+pSB4C5(E-P)</td>
       <td class="row">E23(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">924(25 included)<sup>*</sup></td>
+
       <td class="row">924<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 656: Line 612:
       <td class="row">pTet-RBS34-mRFP-TT in pSB4C5</td>
       <td class="row">pTet-RBS34-mRFP-TT in pSB4C5</td>
       <td class="row">I13521(E-P)+pSB4C5(E-P)</td>
       <td class="row">I13521(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">923(25 included)<sup>*</sup></td>
+
       <td class="row">923<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 678: Line 634:
       <td class="row">pTet-RBS30-aiiA-TT in pSB4C5</td>
       <td class="row">pTet-RBS30-aiiA-TT in pSB4C5</td>
       <td class="row">E24(E-P)+pSB4C5(E-P)</td>
       <td class="row">E24(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">1009+25<sup>*</sup></td>
+
       <td class="row">1034<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 689: Line 645:
       <td class="row">pTet-RBS31-aiiA-TT in pSB4C5</td>
       <td class="row">pTet-RBS31-aiiA-TT in pSB4C5</td>
       <td class="row">E25(E-P)+pSB4C5(E-P)</td>
       <td class="row">E25(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">1008+25<sup>*</sup></td>
+
       <td class="row">1033<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 700: Line 656:
       <td class="row">pTet-RBS32-aiiA-TT in pSB4C5</td>
       <td class="row">pTet-RBS32-aiiA-TT in pSB4C5</td>
       <td class="row">E26(E-P)+pSB4C5(E-P)</td>
       <td class="row">E26(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">1007+25<sup>*</sup></td>
+
       <td class="row">1032<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 711: Line 667:
       <td class="row">pTet-RBS34-aiiA-TT in pSB4C5</td>
       <td class="row">pTet-RBS34-aiiA-TT in pSB4C5</td>
       <td class="row">E27(E-P)+pSB4C5(E-P)</td>
       <td class="row">E27(E-P)+pSB4C5(E-P)</td>
-
       <td class="row">1006+25<sup>*</sup></td>
+
       <td class="row">1031<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 722: Line 678:
       <td class="row">pTet-RBS31-luxI in pSB4C5</td>
       <td class="row">pTet-RBS31-luxI in pSB4C5</td>
       <td class="row">E36(S-P)+E3(X-P)</td>
       <td class="row">E36(S-P)+E3(X-P)</td>
-
       <td class="row">700+25<sup>*</sup></td>
+
       <td class="row">725<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 733: Line 689:
       <td class="row">pTet-RBS32-luxI in pSB4C5</td>
       <td class="row">pTet-RBS32-luxI in pSB4C5</td>
       <td class="row">E36(S-P)+ E4(X-P)</td>
       <td class="row">E36(S-P)+ E4(X-P)</td>
-
       <td class="row">699+25<sup>*</sup></td>
+
       <td class="row">724<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 744: Line 700:
       <td class="row">pTet-RBS30-luxI in pSB4C5</td>
       <td class="row">pTet-RBS30-luxI in pSB4C5</td>
       <td class="row">E36(S-P)+ E2(X-P)</td>
       <td class="row">E36(S-P)+ E2(X-P)</td>
-
       <td class="row">701+25<sup>*</sup></td>
+
       <td class="row">726<sup>*</sup></td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">E. coli MGZ1</td>
       <td class="row">pSB4C5</td>
       <td class="row">pSB4C5</td>
Line 800: Line 756:
<p>
<p>
-
<sup>*</sup> The sequences of these parts contain a 25 bp <a href="http://partsregistry.org/Help:Barcodes">barcode</a>.
+
<sup>*</sup> The sequences of these parts contain at least a 25 bp <a href="http://partsregistry.org/Help:Barcodes">barcode</a>.

Revision as of 10:33, 26 August 2011

UNIPV TEAM 2011

FREEZER


For a detailed correspondence between our BioBrick "wiki names" and submitted BioBrick codes take a look here.

Biobrick name Description Store Length Strain Vector Quality Control Creation Date
BBa_K081022 Plambda-RBS30-luxR-T(B1006)-pLux BioBricks 2011 969 E. coli TOP10 pSB1A2 Seq ok (MIT) 29/06/2011
BBa_C0060 (aiiA) autoinducer inactivation enzyme BioBricks 2011 789* E. coli TOP10 pSB1A2 Seq ok (MIT) 29/06/2011
BBa_B0015 (TT) double terminator 2009 129 E. coli TOP10 pSB1AK3 Seq ok (MIT) FREEZER 2009 Dist
BBa_R0040 (pTet) TetR repressible promoter 2009 54 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_C0061 (luxI) autoinducer synthetase for AHL BioBricks 2011 618* E. coli TOP10 pSB1A2 Seq ok (MIT) 30/06/2011
BBa_I13501 (mRFP-TT) Screening plasmid intermediate requested from the Registry 843* E. coli TOP10 pSB1AK3 Seq ok (MIT) FREEZER Requested 2011
BBa_B0030 RBS30 (eff=0.6) 2009 15 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0031 RBS31 (eff=0.07) derivative of BBa_0030 2009 14 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0032 RBS32 (eff=0.3) 2009 13 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_B0034 RBS34 (eff=1) 2009 12 E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
BBa_I13521 (pTet-RBS34-RFP-TT) Ptet mRFP Constitutive on mRFP 2010 923* E. coli TOP10 pSB1A3 Seq ok (MIT) FREEZER 2010 Dist
BBa_C0261 (RBS34-luxI) AHL-making Enzyme, luxI (+RBS) BioBricks 2011 661* E. coli TOP10 pSB1A2 Seq ok (MIT) 07/07/2011
BBa_I13507 (RBS34-mRFP-TT) Screening plasmid intermediate 2009 861* E. coli TOP10 pSB1A2 Seq ok (MIT) FREEZER 2009 Dist
pSB4C5 Low copy BioBrick standard vector - chloramphenicol antibiotic resistance 2009 3221 E. coli TOP10 pSB4C5 Seq ok (MIT) FREEZER 2009 Dist
pSB3K3 Low to medium copy BioBrick standard vector - kanamycin resistance BioBricks 2011 2750 E. coli TOP10 pSB3K3 Seq ok (MIT) 30/06/2011
BBa_K3000005 GFP (Silver Standard Prefix) with terminator 2010 854 E. coli TOP10 pSB1C3 Seq ok (BMR Gemonics) FREEZER 2010 Ligations


Biobrick name Description Ligation Length Strain Vector Quality Control Creation Date
E1-2 aiiA-TT C0060(E-S)+B0015(E-X) 951* E. coli TOP10 pSB1AK3 Length OK 07/07/2011
E2-2 RBS30-LuxI C0061(X-P)+B0030(S-P) 664* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E3-1 RBS31-LuxI C0061(X-P)+B0031(S-P) 663* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E4-2 RBS32-LuxI C0061(X-P)+B0032(S-P) 662* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E5-2 RBS30-mRFP-TT I13501(X-P)+B0030(S-P) 864* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E6-1 RBS31-mRFP-TT I13501(X-P)+B0031(S-P) 863* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E7-2 RBS32-mRFP-TT I13501(X-P)+B0032(S-P) 862* E. coli TOP10 pSB1A2 Length OK 07/07/2011
E8-3 Plambda-RBS30-luxR-T(B1006)-pLux K081022(E-P)+4C5(E-P) 969* E. coli TOP10 pSB4C5 Length OK 07/07/2011
E9-2 RBS30-aiiA-TT E1(X-P)+B0030(S-P) 972* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E10-1 RBS31-aiiA-TT E1(X-P)+B0031(S-P) 971* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E11-1 RBS32-aiiA-TT E1(X-P)+B0032(S-P) 970* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E12-1 RBS34-aiiA-TT E1(X-P)+ B0034(S-P) 969* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E13-1 pTet-RBS30-LuxI E2(X-P)+R0040(S-P) 726* E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E14 pTet-RBS31-LuxI E3(X-P)+R0040(S-P) 725* E. coli TOP10 pSB1A2 FAILED
E15 pTet-RBS32-LuxI E4(X-P)+R0040(S-P) 724* E. coli TOP10 pSB1A2 FAILED
E16-1 pTet-RBS34-LuxI C0261(X-P)+R0040(S-P) 723* E. coli MGZ1 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E17-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS30-mRFP-TT E5(X-P)+E8(S-P) 1841* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E18-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS31-mRFP-TT E6(X-P)+E8(S-P) 1840* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E19-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS32-mRFP-TT E7(X-P)+E8(S-P) 1839* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E20-2 Plambda-RBS30-luxR-T(B1006)-pLux-RBS34-mRFP-TT I13507(X-P)+E8(S-P) 1838* E. coli TOP10/ E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 13/07/2011
E21-1 pTet-RBS30-mRFP-TT E5(X-P)+R0040(S-P) 926* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E22-2 pTet-RBS31-mRFP-TT E6(X-P)+R0040(S-P) 925* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 13/07/2011
E23-1 pTet-RBS32-mRFP-TT E7(X-P)+R0040(S-P) 924* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 14/07/2011
E24-2 pTet-RBS30-aiiA-TT E9(X-P)+R0040(S-P) 1034* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E25-1 pTet-RBS31-aiiA-TT E10(X-P)+R0040(S-P) 1033* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E26-2 pTet-RBS32-aiiA-TT E11(X-P)+R0040(S-P) 1032* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E27-2 pTet-RBS34-aiiA-TT E12(X-P)+R0040(S-P) 1031* E. coli TOP10 pSB1A2 Seq ok (BMR Genomics) 21/07/2011
E28-1 pTet-RBS30-luxI in pSB4C5 E13(E-P)+pSB4C5(E-P) 726* E. coli MGZ1 pSB4C5 Bad Seq (BMR Genomics) 21/07/2011
E29 pTet-RBS31-luxI in pSB4C5 E14(E-P)+pSB4C5(E-P) 725* E. coli MGZ1 pSB4C5 FAILED
E30 pTet-RBS32-luxI in pSB4C5 E15(E-P)+pSB4C5(E-P) 724* E. coli MGZ1 pSB4C5 FAILED
E31-1 pTet-RBS34-luxI in pSB4C5 E16(E-P)+pSB4C5(E-P) 723* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E32-2 pTet-RBS30-mRFP-TT in pSB4C5 E21(E-P)+pSB4C5(E-P) 926* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E33-2 pTet-RBS31-mRFP-TT in pSB4C5 E22(E-P)+pSB4C5(E-P) 925* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E34-1 pTet-RBS32-mRFP-TT in pSB4C5 E23(E-P)+pSB4C5(E-P) 924* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E35-2 pTet-RBS34-mRFP-TT in pSB4C5 I13521(E-P)+pSB4C5(E-P) 923* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 21/07/2011
E36 pTet-J61002 in pSB4C5 pTet-J61002(E-P)+pSB4C5(E-P) 923* E. coli TOP10 pSB4C5 25/07/2011
E37-2 pTet-RBS30-aiiA-TT in pSB4C5 E24(E-P)+pSB4C5(E-P) 1034* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E38-1 pTet-RBS31-aiiA-TT in pSB4C5 E25(E-P)+pSB4C5(E-P) 1033* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E39-1 pTet-RBS32-aiiA-TT in pSB4C5 E26(E-P)+pSB4C5(E-P) 1032* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E40-2 pTet-RBS34-aiiA-TT in pSB4C5 E27(E-P)+pSB4C5(E-P) 1031* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E41N-1 pTet-RBS31-luxI in pSB4C5 E36(S-P)+E3(X-P) 725* E. coli MGZ1 pSB4C5 04/08/2011
E42-1 pTet-RBS32-luxI in pSB4C5 E36(S-P)+ E4(X-P) 724* E. coli MGZ1 pSB4C5 Seq ok (BMR Genomics) 29/07/2011
E43-? pTet-RBS30-luxI in pSB4C5 E36(S-P)+ E2(X-P) 726* E. coli MGZ1 pSB4C5 ??? 25/08/2011
J101-E5 J23101-RBS30-mRFP-TT in pSB4C5 BBa_J23101(S-P)+ E5(S-P) 905* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
J101-31 J23101-RBS31-mRFP-TT in pSB4C5 BBa_J23101(S-P)+ E6(S-P) 904* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
J101-E7 J23101-RBS32-mRFP-TT in pSB4C5 BBa_J23101(S-P)+ E7(S-P) 903* E. coli MGZ1 pSB4C5 Length OK 02/08/2011
J101-4C5 J23101-RBS34-mRFP-TT in pSB4C5 BBa_J23101(E-P)+ pSB4C5(E-P) 902* E. coli MGZ1 pSB4C5 Length OK 02/08/2011

* The sequences of these parts contain at least a 25 bp barcode.

Laboratory for Biomedical Informatics | Centre of Tissutal Engineering

University of Pavia
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