Team:UNIPV-Pavia

From 2011.igem.org

(Difference between revisions)
 
(67 intermediate revisions not shown)
Line 1: Line 1:
{{main}}
{{main}}
<html>
<html>
-
  <h2 class="art-postheader">
+
 
-
SAFETY
+
<table align='center' width='100%'>
-
</h2>
+
<tr>
 +
<td>
 +
<div style='text-align:center'><div class="thumbinner" style="width:100%;">
 +
<img alt="" src="https://static.igem.org/mediawiki/2011/b/b1/UNIPV_Pavia_Work_in_progress_scribblings.jpg" class="thumbimage" width="93%"></a></div></div>
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h2 class="art-postheader">The project</h2>
<div class="cleared"></div>
<div class="cleared"></div>
<div class="art-postcontent">
<div class="art-postcontent">
-
<p>Enter Page content here...</p>
+
<p align='justify'>Our work aims at implementing the engineering concept of closed-loop control in <em>E. coli</em>, exploiting quorum sensing. As a proof of concept, we designed a simple genetic controller that regulates the concentration of 3OC6-HSL signalling molecule around  a user-defined set-point. The controlled variable (3OC6-HSL) increases as a function of the exogenous anhydroTetracycline input, that triggers LuxI expression. The controller senses the 3OC6-HSL concentration and activates the production of AiiA, that degrades it.
-
<p style="text-align:left;">
+
To observe the desired behaviour, a fine tuning of the system was necessary.  The transcriptional/translational strength of the regulatory elements (promoter+RBS in several combinations) and the enzyme activities were measured and exploited to identify a mathematical model able to predict the behaviour of the controlled system. These predictions made possible an in silico rational fine tuning of the circuit: the most promising modules were selected and assembled into the final circuit, avoiding a cost and time expensive combinatorial approach.
 +
</p>
 +
<br>
 +
 
 +
 
 +
 
 +
 
 +
<link rel="stylesheet" href="https://2011.igem.org/Team:UNIPV-Pavia/Templates/nivo_default_css?action=raw&ctype=text/css" type="text/css"/>
 +
<link rel="stylesheet" href="https://2011.igem.org/Team:UNIPV-Pavia/Templates/nivo_css?action=raw&ctype=text/css" type="text/css"/>
 +
<script type="text/javascript" src="https://2011.igem.org/Team:UNIPV-Pavia/Templates/jquery_js?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2011.igem.org/Team:UNIPV-Pavia/Templates/nivo_slider_pack?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript">
 +
    $(window).load(function() {
 +
        $('#slider').nivoSlider({
 +
effect:'fade',
 +
pauseOnHover:true
 +
});
 +
    });
 +
</script>
 +
 
 +
 
 +
 
 +
 
 +
<div style="width:550px;margin:10px auto;">
 +
<div class="slider-wrapper theme-default">
 +
<div class="ribbon"></div>
 +
 
 +
<div id="nivoslider-125" class="nivoSlider" style="width:550px;height:366px;">
 +
 
 +
<img src="https://static.igem.org/mediawiki/2011/2/2d/UNIPV_Hometeam1.jpg" title="UNIPV iGEM team" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/0/0a/UNIPV_Hometeam2.jpg" title="UNIPV iGEM team" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/e/e1/UNIPV_Hometeam3.jpg" title="UNIPV iGEM team" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/f/f2/UNIPV_Hometeam4.jpg" title="Work in progress" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/9/9b/UNIPV_Home1.jpg" title="Pavia and the river Ticino" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/a/a2/UNIPV_Home2.jpg" title="University of Pavia" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/f/f7/UNIPV_Home3.jpg" title="Faculty of Engineering" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/f/f0/UNIPV_Home4.jpg" title="Scientific campus" alt="" />
 +
 
 +
</div>
 +
</div>
 +
<div id="nivoslider-125-caption-0" class="nivo-html-caption">You can add captions too&#8230;</div>
 +
<p><script type="text/javascript">
 +
jQuery(window).load(function(){
 +
    jQuery("#nivoslider-125").nivoSlider({
 +
        effect:"random",
 +
        slices:15,
 +
        boxCols:8,
 +
        boxRows:4,
 +
        animSpeed:500,
 +
        pauseTime:3000,
 +
        startSlide:0,
 +
        directionNav:true,
 +
        directionNavHide:true,
 +
        controlNav:true,
 +
        controlNavThumbs:false,
 +
        controlNavThumbsFromRel:true,
 +
        keyboardNav:true,
 +
        pauseOnHover:true,
 +
        manualAdvance:false
 +
    });
 +
});
 +
</script>
 +
</div>
 +
 
 +
<br><br>
 +
 
-
<p style="text-align:justify;">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Etiam pharetra, tellus sit amet congue vulputate, nisi erat iaculis nibh, vitae feugiat sapien ante eget mauris. Cras elit nisl, rhoncus nec iaculis ultricies, feugiat eget sapien. Pellentesque ac felis tellus.</p>
 
-
<p style="text-align:justify;">Aenean sollicitudin imperdiet arcu, vitae dignissim est posuere id. Duis placerat justo eu nunc interdum ultrices. Phasellus elit dolor, porttitor id consectetur sit amet, posuere id magna. Lorem ipsum dolor sit amet, consectetur adipiscing elit.</p>
 
-
<p style="text-align:justify;"> Suspendisse pharetra auctor pharetra. Nunc a sollicitudin est. Curabitur ullamcorper gravida felis, sit amet scelerisque lorem iaculis sed. Donec vel neque in neque porta venenatis sed sit amet lectus. Fusce ornare elit nisl, feugiat bibendum lorem. Vivamus pretium dictum sem vel laoreet. In fringilla pharetra purus, semper vulputate ligula cursus in. Donec at nunc nec dui laoreet porta eu eu ipsum. Sed eget lacus sit amet risus elementum ZZZZZZ.</p>
 
</html>
</html>
{{end}}
{{end}}

Latest revision as of 13:14, 19 September 2011

UNIPV TEAM 2011


The project

Our work aims at implementing the engineering concept of closed-loop control in E. coli, exploiting quorum sensing. As a proof of concept, we designed a simple genetic controller that regulates the concentration of 3OC6-HSL signalling molecule around a user-defined set-point. The controlled variable (3OC6-HSL) increases as a function of the exogenous anhydroTetracycline input, that triggers LuxI expression. The controller senses the 3OC6-HSL concentration and activates the production of AiiA, that degrades it. To observe the desired behaviour, a fine tuning of the system was necessary. The transcriptional/translational strength of the regulatory elements (promoter+RBS in several combinations) and the enzyme activities were measured and exploited to identify a mathematical model able to predict the behaviour of the controlled system. These predictions made possible an in silico rational fine tuning of the circuit: the most promising modules were selected and assembled into the final circuit, avoiding a cost and time expensive combinatorial approach.


You can add captions too…



Retrieved from "http://2011.igem.org/Team:UNIPV-Pavia"