Team:UNICAMP-EMSE Brazil/Notebook/14 September 2011
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:*Preparing our sequences to insert into pSB1C3 and ship to MIT | :*Preparing our sequences to insert into pSB1C3 and ship to MIT | ||
'''Samples:''' | '''Samples:''' | ||
- | [[Image:UNICAMP-EMSE Brazil Gel2 14 09 11.jpg|thumb|right|300px|<font color: blue>'''Electrophoresis Gel | + | [[Image:UNICAMP-EMSE Brazil Gel2 14 09 11.jpg|thumb|right|300px|<font color: blue>'''Electrophoresis Gel 2, 14/09/2011.'''</font> <br>Gel 2 upper part: Ladder,flhDC C1,flhDC C2,flhDC C3, flhDC C4, SoxR C1, SoxR C2, SoxR C3, HlyA C1, HlyA C8, HlyA C9, HlyB(?) C1, HlyB(?) C2, HlyB(?) C3, HlyB(?) C7, <br> Bottom: Ladder, Qse C1, Qse C4, IL-12 C1, IL-12 C2 ]] |
:*'''Gel 2, upper part:''' | :*'''Gel 2, upper part:''' |
Latest revision as of 11:17, 17 September 2011
Home | Project | Methods | Results | Data | Team | Notebook | Human Practices | Safety | Profile | Sponsors | Wix |
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Notebook
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14 September 2011
Isolate and cut inserts
Objective:
- Isolate and cut the inserts (volume of ladder applied 6uL; checking digestion sample: 40uL digested sample +4uL buffer )
Samples:
- Gel 1:
- SoxS+RBS+IL-10 (E-S)
- Ladder:
- 100-10.000 bp (Fermentas)
- Gel Agarose concentration:
- 1,0%
- Results:
Construction | Total size (gene+vector) | Linear vector size | Gene size | Result |
---|---|---|---|---|
SoxS+RBS+IL-10 | 3448 bp | 2800 bp | 648 bp | Digestion and size OK |
Preparing sequences to shipping
Objective:
- Preparing our sequences to insert into pSB1C3 and ship to MIT
Samples:
- Gel 2, upper part:
- Ladder | flhDC C1 | flhDC C2 | flhDC C3 | flhDC C4 | SoxR C1 | SoxR C2 | SoxR C3 | HlyA C1 | HlyA C8 | HlyA C9 | HlyB(?) C1 | HlyB(?) C2 | HlyB(?) C3 | HlyB(?) C7 |
- Gel2, bottom part:
- Ladder | Qse C1 | Qse C4 | IL-12 C1 | IL-12 C2
- Ladder:
- 100-10.000 bp (Fermentas)
- Gel Agarose concentration:
- 1,0%
- Results:
Construction | Total size (gene+vector) | Linear vector size | Gene size | Result |
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