Team:UNICAMP-EMSE Brazil/Notebook/14 September 2011
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+ | ==='''Preparing sequences to shipping'''=== | ||
+ | '''Objective:''' | ||
+ | :*Preparing our sequences to insert into pSB1C3 and ship to MIT | ||
+ | '''Samples:''' | ||
+ | [[Image:UNICAMP-EMSE Brazil Gel2 14 09 11.jpg|thumb|right|300px|<font color: blue>'''Electrophoresis Gel 1, 14/09/2011.'''</font> <br>Gel 2 upper part: Ladder | flhDC C1 | flhDC C2 | flhDC C3 | flhDC C4 | SoxR C1 | SoxR C2 | SoxR C3 | HlyA C1 | HlyA C8 | HlyA C9 | HlyB(?) C1 | HlyB(?) C2 | HlyB(?) C3 | HlyB(?) C7 | <br> Bottom: Ladder | Qse C1 | Qse C4 | IL-12 C1 | IL-12 C2]] | ||
+ | |||
+ | :*'''Gel 2, upper part:''' | ||
+ | ::*Ladder | flhDC C1 | flhDC C2 | flhDC C3 | flhDC C4 | SoxR C1 | SoxR C2 | SoxR C3 | HlyA C1 | HlyA C8 | HlyA C9 | HlyB(?) C1 | HlyB(?) C2 | HlyB(?) C3 | HlyB(?) C7 | | ||
+ | :*'''Gel2, bottom part:''' | ||
+ | ::*Ladder | Qse C1 | Qse C4 | IL-12 C1 | IL-12 C2 | ||
+ | |||
+ | *'''Ladder:''' | ||
+ | :*100-10.000 bp (Fermentas) | ||
+ | *'''Gel Agarose concentration:''' | ||
+ | :*1,0% | ||
+ | |||
+ | <br clear="all" /> | ||
+ | *'''<font size=+5 color=teal>Results:''' | ||
+ | </font> | ||
+ | <font align=center> | ||
+ | {|border="1" cellpadding="5" cellspacing="0" align="center" | ||
+ | |- | ||
+ | ! scope="col" style="background:#efefef;" | Construction | ||
+ | ! scope="col" style="background:#efefef;" | Total size (gene+vector) | ||
+ | ! scope="col" style="background:#efefef;" | Linear vector size | ||
+ | ! scope="col" style="background:#efefef;" | Gene size | ||
+ | ! scope="col" style="background:#efefef;" | Result | ||
+ | |||
+ | |}</font> | ||
+ | <br> | ||
+ | <br> | ||
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<img src="https://static.igem.org/mediawiki/2011/c/cb/UNICAMP-EMSE_Brazil_Stresswars.png" width="893px"></img> | <img src="https://static.igem.org/mediawiki/2011/c/cb/UNICAMP-EMSE_Brazil_Stresswars.png" width="893px"></img> | ||
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Revision as of 11:14, 17 September 2011
Home | Project | Methods | Results | Data | Team | Notebook | Human Practices | Safety | Profile | Sponsors | Wix |
Contents |
Notebook
Click on a date to see what we have done!
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14 September 2011
Isolate and cut inserts
Objective:
- Isolate and cut the inserts (volume of ladder applied 6uL; checking digestion sample: 40uL digested sample +4uL buffer )
Samples:
- Gel 1:
- SoxS+RBS+IL-10 (E-S)
- Ladder:
- 100-10.000 bp (Fermentas)
- Gel Agarose concentration:
- 1,0%
- Results:
Construction | Total size (gene+vector) | Linear vector size | Gene size | Result |
---|---|---|---|---|
SoxS+RBS+IL-10 | 3448 bp | 2800 bp | 648 bp | Digestion and size OK |
Preparing sequences to shipping
Objective:
- Preparing our sequences to insert into pSB1C3 and ship to MIT
Samples:
- Gel 2, upper part:
- Ladder | flhDC C1 | flhDC C2 | flhDC C3 | flhDC C4 | SoxR C1 | SoxR C2 | SoxR C3 | HlyA C1 | HlyA C8 | HlyA C9 | HlyB(?) C1 | HlyB(?) C2 | HlyB(?) C3 | HlyB(?) C7 |
- Gel2, bottom part:
- Ladder | Qse C1 | Qse C4 | IL-12 C1 | IL-12 C2
- Ladder:
- 100-10.000 bp (Fermentas)
- Gel Agarose concentration:
- 1,0%
- Results:
Construction | Total size (gene+vector) | Linear vector size | Gene size | Result |
---|