Team:Tokyo Metropolitan/Notebook/S09

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-Targeting-

-Plasmid extraction and DNA refining

Extracted plasmid and refined DNA used Cell Biochemical Lab's kits for No. 29 of J23100-K317037 and No. 19 of J23100-P0440.


Results measured concentration

No. 29 of J23100-K317037 was 150.

No. 19 of J23100-P0440 was 138.


J22005

I. 105

II. 155

III. 62


concentration was ng/ul


-Treat restriction enzyme

J23100-K317037 -> S and P

J23100-P0440 -> E and P

K283030 -> X and P

J22005 -> N

pSB1C3 -> E and P

incubated at 37 deg C after treat with the preceding enzymes.


-Setrilization for liquid medium

Setrilised for nine tubes 4 ml.


-Visualization-

-Plasmid extraction

Promoter

Suppressed expression system

RFP


-DNA refinig

Above samples




@plasmid extraction, DNA refine, DNA densitometry, restrictin emzyme(EcoRⅠ, SpeⅠ), electrophoresis OriTr-GFP-OriTr

@excess preculture medium move to media(Amp).