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Team:Tokyo Metropolitan/Notebook/S05
From 2011.igem.org
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Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI | Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI | ||
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| + | = -Visualization- = | ||
| + | -Ligation | ||
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| + | J23100-I714051 | ||
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| + | |||
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| + | -Transformation | ||
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| + | J23100-I714051 | ||
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| + | |||
| + | -Plasmid extraction | ||
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| + | Promoter and suppressed expression system | ||
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| + | |||
| + | -DNA refining | ||
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| + | Promoter and suppressed expression system | ||
Latest revision as of 13:20, 5 October 2011
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-Speeding up-
Digested HNS(wt) with XbaI+SpeI and I20260 with BlnⅠ.
Although concentration was very low, try to ligate HNS(wt) +I20260 and induce them into competent cell.
Amplified and purification I20260 with PCR.
Digestion HNS(wt) with XbaI+SpeI, I20260 with BlnI
-Visualization-
-Ligation
J23100-I714051
-Transformation
J23100-I714051
-Plasmid extraction
Promoter and suppressed expression system
-DNA refining
Promoter and suppressed expression system
