-Visualization-

Maked liquid medium.

100ml

Yeast extract 0.5 g

Peptone 1 g

NaCl 1 g

Amp 100 µl

According to protocol.

Did not sterilize before autoclave, just in the bottle.

Add Amp and use for pre-culture.

Maked agarose gel.

Agarose 1.2 g

TAE Buffer 100 ml

1. Melted agarose and TAE Buffer in conical flask by microwave oven.

2. Poured into a mould pierced a comb.

3. After set kept in TAE Buffer.

Pre cultured colony of GFP + OriTr.

-Targeting-

1. Maked liquid medium.

2. Checked transformation.

-> J23100 and K317037 were grown.

3. Plasmid extraction

-> K283030 extracted. Keep the extract in the coldroom in Cell-biochemistry lab.

4. Pre culture

Each of J23100 and K317037 cultured three.

They have been incubating at 37 deg C.