Team:Paris Bettencourt/Data

From 2011.igem.org

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<h1>Data page</h1>
<h1>Data page</h1>
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<h2>The YFP concentration system : YFP:tetR and TetO array</h2>
+
<h2>The YFP concentration system</h2>
</html>
</html>
-
[[File:YFP_concentration_principle.jpg|center|950px|The YFP concentration system principle]]
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[[File:YFP_concentration_principle.jpg|center|The YFP concentration system principle]]
<html>
<html>
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<h3>Data for our favorite new parts</h3>
<h3>Data for our favorite new parts</h3>
</html>
</html>
 +
# [http://partsregistry.org/Part:BBa_K606025 Main Page] - '''YFP:tetR recombinant fusion protein, BBa_K606025''':  It's a recombinant fusion protein composed of Yellow Fluorescent Protein (YFP) and Tetracycline Repressor Protein (tetR) that can bind to the tet operator sequence (tetO).
# [http://partsregistry.org/Part:BBa_K606026 Main Page] - '''TetO Array, BBa_K606026''': 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.  
# [http://partsregistry.org/Part:BBa_K606026 Main Page] - '''TetO Array, BBa_K606026''': 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.  
-
# [http://partsregistry.org/Part:BBa_K606003 Main Page] - '''Multihost replicative plasmid pHM3, BBa_K606003''': This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
+
# [http://partsregistry.org/Part:BBa_K606027 Main Page] - '''pVeg+SpoVG- YFP:tetR- Double Terminator , BBa_K606027''': The YFP:tetR expression system.
-
# [http://partsregistry.org/Part:BBa_K606025 Main Page] - '''YFP:tetR recombinant fusion protein, BBa_K606025''': This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
+
<html>
<html>
<h3>Data for Pre-existing Parts</h3>
<h3>Data for Pre-existing Parts</h3>
</html>
</html>
-
# [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - '''pVeg + SpoVG, BBa_K143053''' (Imperial College, iGEM 2008):  
+
# [http://partsregistry.org/Part:BBa_K143053 Main Page] - '''pVeg + SpoVG, BBa_K143053''': promoter and RBS for B. subtilis (Imperial College, iGEM 2008)  
 +
# [http://partsregistry.org/Part:BBa_B0015 Main Page] - '''Double standard terminator, BBa_B0015''': standard double terminator<html>
<html>
<html>
-
<br/>
+
 
-
<br/>
+
<ul>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/GFPLac_diffusion">See the design</a></li>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion">See the experiments</a></li>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion_experiments">See the nanotubes experiments</a></li>
 +
</ul>
 +
 
<h2>The T7 polymerase diffusion system</h2>
<h2>The T7 polymerase diffusion system</h2>
</html>
</html>
-
[[File:T7_pol_diff.jpg|center|950px|The T7 polymerase diffusion system principle]]
+
[[File:T7_pol_diff.jpg|center|The T7 polymerase diffusion system principle]]
<html>
<html>
-
<h3>Data for Pre-existing Parts</h3>
+
<h3>Data for our favorite new parts</h3>
</html>
</html>
 +
# [http://partsregistry.org/Part:BBa_K606039:Experience Experience] - '''pVeg SpoVG T7 SpoVG RFP double terminator, BBa_K606039''': T7 and RFP under constitutive promoter pVeg + SpoVG, emitter system to “send” the T7 polymerase.
 +
# [http://partsregistry.org/Part:BBa_K606036:Experience Experience] - '''pT7 SpoVG T7 SpoVG GFP T7terminator, BBa_K606036''': T7 and GFP under a pT7 promoter to response a emission of T7.
 +
<html>
 +
 +
<ul>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/T7_diffusion">See the design</a></li>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Modeling/T7_diffusion">See the model</a></li>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/T7_diffusion">See the experiments</a></li>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/T7_diffusion_experiments">See the nanotubes experiments</a></li>
 +
</ul>
 +
 +
 +
<h2>The tRNA Amber diffusion system</h2>
 +
 +
</html>
 +
[[File:tRNA_amber_principle.jpg|center|The tRNA Amber diffusion system principle]]
 +
<html>
<h3>Data for our favorite new parts</h3>
<h3>Data for our favorite new parts</h3>
</html>
</html>
-
# [http://partsregistry.org/Part:BBa_K606026 Main Page] - '''TetO Array, BBa_K606026''': 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.  
+
# [http://partsregistry.org/Part:BBa_K606040 Main Page] - '''Promoter Hyperspank, BBa_K606040''': IPTG Inducible promoter shortened compared to the one that was before in the registry.  
-
# [http://partsregistry.org/Part:BBa_K606003 Main Page] - '''Multihost replicative plasmid pHM3, BBa_K606003''': This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
+
# [http://partsregistry.org/Part:BBa_K606041 Main Page] - '''pHyperspank Tyrosine amber supressor tRNA double terminator, BBa_K606041''': system for using the tRNA in a IPTG inducible system.
-
# [http://partsregistry.org/Part:BBa_K606025 Main Page] - '''YFP:tetR recombinant fusion protein, BBa_K606025''': This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
+
# [http://partsregistry.org/Part:BBa_K606034 Main Page] - '''Tyrosine amber supressor tRNA for subtilis, BBa_K606034''': tRNA tyrosine amber compatible with both B. subtilis and E. coli.
 +
# [http://partsregistry.org/Part:BBa_K606032 Main Page] - '''T7 amber, BBa_K606032''': This T7 polymerase contains two amber mutation to be a good receiver in a emitter/receiver system with a Tyrosine tRNA amber.
 +
# [http://partsregistry.org/Part:BBa_K606064 Main Page] - '''T7 amber with autoamplification loop, BBa_K606064'''
 +
# [http://partsregistry.org/Part:BBa_K606065 Main Page] - '''IPTG inducible tRNA Amber emitter with RFP monitor, BBa_K606065'''
 +
# [http://partsregistry.org/Part:BBa_K606067 Main Page] - '''Upstream LacI expression IPTG inducible tRNA emitter with RFP Monitor, BBa_K606067'''
<html>
<html>
<h3>Data for Pre-existing Parts</h3>
<h3>Data for Pre-existing Parts</h3>
</html>
</html>
-
# [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - '''pVeg + SpoVG, BBa_K143053''' (Imperial College, iGEM 2008):  
+
# [http://partsregistry.org/Part:BBa_K143053 Main Page] - '''pVeg + SpoVG, BBa_K143053''': promoter and RBS for B. subtilis (Imperial College, iGEM 2008)  
 +
# [http://partsregistry.org/Part:BBa_B0015 Main Page] - '''Double standard terminator, BBa_B0015'''
<html>
<html>
<br/>
<br/>
 +
 +
<ul>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/tRNA_diffusion">See the design</a></li>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Modeling/tRNA_diffusion">See the model</a></li>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/tRNA_diffusion">See the experiments</a></li>
 +
</ul>
 +
<br/>
<br/>
 +
<h2>The ComS diffusion system</h2>
-
<h2>The tRNA Amber diffusion system</h2>
+
</html>
 +
[[File:coms_diffusion_principle.jpg|center|The ComS diffusion system principle]]
 +
<html>
 +
<h3>Data for our favorite new parts</h3>
</html>
</html>
-
[[File:tRNA_amber_principle.jpg|center|950px|The tRNA Amber diffusion system principle]]
+
# [http://partsregistry.org/Part:BBa_K606037 Main Page] - '''ComS gene, BBa_K606037''': The comS gene can trigger the MecA/ComS/ComK system. The start and stop codon has been changed for more standard codons.
 +
# [http://partsregistry.org/Part:BBa_K606038 Main Page] - '''ComS gene Codon optimized, BBa_K606038''': This is the same gene than the previous one, but it has been synthetized de novo.
<html>
<html>
<h3>Data for Pre-existing Parts</h3>
<h3>Data for Pre-existing Parts</h3>
</html>
</html>
 +
# [http://partsregistry.org/Part:BBa_K143053 Main Page] - '''pVeg + SpoVG, BBa_K143053''': promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
 +
# [http://partsregistry.org/Part:BBa_B0015 Main Page] - '''Double standard terminator, BBa_B0015''': standard double terminator
 +
# [http://partsregistry.org/Part:BBa_K143021 Main Page] - '''RFP, BBa_K143021''': RFP m-cherry.
 +
# '''Elowitz's pComG-CFP/pComS-YFP reporter strain''' - Competence reporter strain. Published in this paper [http://www.elowitz.caltech.edu/publications/Tunability.pdf]
 +
# '''CodY- strain''' - From Abraham L. Sonenshein's laboratory, Tufts university [http://www.tufts.edu/sackler//facultyIntros/sonensheinA.html]
 +
 +
<html>
 +
 +
<ul>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/ComS_diffusion">See the design</a></li>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Modeling/ComS_diffusion">See the model</a></li>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/ComS_diffusion">See the experiments</a></li>
 +
</ul>
 +
 +
 +
<h2>The SinOp system</h2>
-
<h3>Data for our favorite new parts</h3>
 
</html>
</html>
-
# [http://partsregistry.org/Part:BBa_K606026 Main Page] - '''TetO Array, BBa_K606026''': 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
+
[[File:sinop_principle.jpg|center|The SinOp system principle]]
-
# [http://partsregistry.org/Part:BBa_K606003 Main Page] - '''Multihost replicative plasmid pHM3, BBa_K606003''': This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
+
-
# [http://partsregistry.org/Part:BBa_K606025 Main Page] - '''YFP:tetR recombinant fusion protein, BBa_K606025''': This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
+
<html>
<html>
 +
<h3>Data for our favorite new parts</h3>
 +
</html>
 +
# [http://partsregistry.org/Part:BBa_K606046 Main Page] - '''KinA gene, BBa_K606046''' KinA gene. SinOp kinase.
 +
# [http://partsregistry.org/Part:BBa_K606047 Main Page] - '''pVeg-SpoVg-KinA gene, BBa_K606047''' KinA gene with B. subtilis promoter
 +
<html>
<h3>Data for Pre-existing Parts</h3>
<h3>Data for Pre-existing Parts</h3>
</html>
</html>
-
# [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - '''pVeg + SpoVG, BBa_K143053''' (Imperial College, iGEM 2008):
+
# '''SinOp reporter strain''', given by J.W. Veening, published in this paper [http://jb.asm.org/cgi/content/full/192/8/2053/DC1]
<html>
<html>
-
<br/>
 
-
<br/>
 
-
<h2>The ComS diffusion system</h2>
+
<ul>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/SinOp">See the design</a></li>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/SinOp">See the experiments</a></li>
 +
</ul>
 +
 
 +
 
 +
<h2>The lambda switch system</h2>
</html>
</html>
-
[[File:coms_diffusion_principle.jpg|center|950px|The ComS diffusion system principle]]
+
[[File:lambda_switch_principle.jpg|center|The lambda switch system principle]]
<html>
<html>
-
<h3>Data for Pre-existing Parts</h3>
+
<h3>Data for our favorite new parts</h3>
</html>
</html>
 +
# [http://partsregistry.org/Part:BBa_K606044 Main Page] - '''pVeg SpoVg CI TT, BBa_K606044''': Expression casette for for CI in B. subtilis.
 +
# [http://partsregistry.org/Part:BBa_K606068 Main Page] - '''pVeg SpoVg CI SpoVG RFP TT, BBa_K606068''': Expression casette for for CI in B. subtilis with RFP monitor.
 +
<html>
-
<h3>Data for our favorite new parts</h3>
+
<h3>Data for Pre-existing Parts</h3>
</html>
</html>
-
# [http://partsregistry.org/Part:BBa_K606026 Main Page] - '''TetO Array, BBa_K606026''': 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.  
+
# [http://partsregistry.org/Part:BBa_K143053 Main Page] - '''pVeg + SpoVG, BBa_K143053''': promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
-
# [http://partsregistry.org/Part:BBa_K606003 Main Page] - '''Multihost replicative plasmid pHM3, BBa_K606003''': This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
+
# [http://partsregistry.org/Part:BBa_B0015 Main Page] - '''Double standard terminator, BBa_B0015''': standard double terminator
-
# [http://partsregistry.org/Part:BBa_K606025 Main Page] - '''YFP:tetR recombinant fusion protein, BBa_K606025''': This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
+
# [http://partsregistry.org/Part:BBa_C0052 Main Page] - '''CI, BBa_B0052''' - CI gene from the lambda phage.
<html>
<html>
 +
<br/>
 +
<ul>
 +
<li><a href="https://2011.igem.org/Team:Paris_Bettencourt/Lambda_switch">See the design</a></li>
 +
</ul>
 +
 +
<br/>
 +
 +
<h2>GFP diffusion</h2>
 +
</html>
 +
[[File:Nanotube_and_GFP.jpeg|center|600px]]
 +
 +
<html>
<h3>Data for Pre-existing Parts</h3>
<h3>Data for Pre-existing Parts</h3>
 +
</html>
</html>
-
# [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - '''pVeg + SpoVG, BBa_K143053''' (Imperial College, iGEM 2008):
+
# [http://partsregistry.org/Part:BBa_K143079 Experience] - '''Pveg-spoVG GFP expression construct, BBa_K143079''' (Imperial College, iGEM 2008)
<html>
<html>
 +
 +
<br/>
<br/>
<br/>
<br/>

Latest revision as of 03:07, 29 October 2011

Team IGEM Paris 2011

Data page

The YFP concentration system

The YFP concentration system principle

Data for our favorite new parts

  1. Main Page - YFP:tetR recombinant fusion protein, BBa_K606025: It's a recombinant fusion protein composed of Yellow Fluorescent Protein (YFP) and Tetracycline Repressor Protein (tetR) that can bind to the tet operator sequence (tetO).
  2. Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  3. Main Page - pVeg+SpoVG- YFP:tetR- Double Terminator , BBa_K606027: The YFP:tetR expression system.

Data for Pre-existing Parts

  1. Main Page - pVeg + SpoVG, BBa_K143053: promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
  2. Main Page - Double standard terminator, BBa_B0015: standard double terminator

    The T7 polymerase diffusion system

The T7 polymerase diffusion system principle

Data for our favorite new parts

  1. Experience - pVeg SpoVG T7 SpoVG RFP double terminator, BBa_K606039: T7 and RFP under constitutive promoter pVeg + SpoVG, emitter system to “send” the T7 polymerase.
  2. Experience - pT7 SpoVG T7 SpoVG GFP T7terminator, BBa_K606036: T7 and GFP under a pT7 promoter to response a emission of T7.

The tRNA Amber diffusion system

The tRNA Amber diffusion system principle

Data for our favorite new parts

  1. Main Page - Promoter Hyperspank, BBa_K606040: IPTG Inducible promoter shortened compared to the one that was before in the registry.
  2. Main Page - pHyperspank Tyrosine amber supressor tRNA double terminator, BBa_K606041: system for using the tRNA in a IPTG inducible system.
  3. Main Page - Tyrosine amber supressor tRNA for subtilis, BBa_K606034: tRNA tyrosine amber compatible with both B. subtilis and E. coli.
  4. Main Page - T7 amber, BBa_K606032: This T7 polymerase contains two amber mutation to be a good receiver in a emitter/receiver system with a Tyrosine tRNA amber.
  5. Main Page - T7 amber with autoamplification loop, BBa_K606064
  6. Main Page - IPTG inducible tRNA Amber emitter with RFP monitor, BBa_K606065
  7. Main Page - Upstream LacI expression IPTG inducible tRNA emitter with RFP Monitor, BBa_K606067

Data for Pre-existing Parts

  1. Main Page - pVeg + SpoVG, BBa_K143053: promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
  2. Main Page - Double standard terminator, BBa_B0015



The ComS diffusion system

The ComS diffusion system principle

Data for our favorite new parts

  1. Main Page - ComS gene, BBa_K606037: The comS gene can trigger the MecA/ComS/ComK system. The start and stop codon has been changed for more standard codons.
  2. Main Page - ComS gene Codon optimized, BBa_K606038: This is the same gene than the previous one, but it has been synthetized de novo.

Data for Pre-existing Parts

  1. Main Page - pVeg + SpoVG, BBa_K143053: promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
  2. Main Page - Double standard terminator, BBa_B0015: standard double terminator
  3. Main Page - RFP, BBa_K143021: RFP m-cherry.
  4. Elowitz's pComG-CFP/pComS-YFP reporter strain - Competence reporter strain. Published in this paper [1]
  5. CodY- strain - From Abraham L. Sonenshein's laboratory, Tufts university [2]

The SinOp system

The SinOp system principle

Data for our favorite new parts

  1. Main Page - KinA gene, BBa_K606046 KinA gene. SinOp kinase.
  2. Main Page - pVeg-SpoVg-KinA gene, BBa_K606047 KinA gene with B. subtilis promoter

Data for Pre-existing Parts

  1. SinOp reporter strain, given by J.W. Veening, published in this paper [3]

The lambda switch system

The lambda switch system principle

Data for our favorite new parts

  1. Main Page - pVeg SpoVg CI TT, BBa_K606044: Expression casette for for CI in B. subtilis.
  2. Main Page - pVeg SpoVg CI SpoVG RFP TT, BBa_K606068: Expression casette for for CI in B. subtilis with RFP monitor.

Data for Pre-existing Parts

  1. Main Page - pVeg + SpoVG, BBa_K143053: promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
  2. Main Page - Double standard terminator, BBa_B0015: standard double terminator
  3. Main Page - CI, BBa_B0052 - CI gene from the lambda phage.



GFP diffusion

Nanotube and GFP.jpeg

Data for Pre-existing Parts

  1. Experience - Pveg-spoVG GFP expression construct, BBa_K143079 (Imperial College, iGEM 2008)



Retrieved from "http://2011.igem.org/Team:Paris_Bettencourt/Data"