Team:Osaka/week13

From 2011.igem.org

Revision as of 15:22, 24 October 2011 by Toshi (Talk | contribs)


October 23 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Electrophoresis of digests
  4. Gel extraction
  5. Ligation
    • 142(C):126(upstream)+118(downstream)+1-3A(vector)
  6. To liquid culture:130,131,132,133,134,138,139,140,141
  7. Transformation of ligation products

October 24 (Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Electrophoresis of digests
    • 131,133,134 x
  4. Gel extraction
  5. Ligation
    • 143(C):132(upstream)+118(downstream)+1-3A(vector)
  6. To liquid culture:131,133,134,142
  7. Plating (Tolerance assay)
  8. Transformation of ligation products

October 25 (Tue)

October 26 (Wed)

October 27 (Thu)

October 28 (Fri)

October 29 (Sat)

Back to Notebook

week 1|2|3|4|5|6|7|8|9|10|11|12|13

Retrieved from "http://2011.igem.org/Team:Osaka/week13"