Team:Lyon-INSA-ENS/Realisation/Week5

From 2011.igem.org

Revision as of 14:57, 26 July 2011 by Ahaag (Talk | contribs)




Week 5


From Monday the 4th of July to Friday the 8th of July 2011







Monday


Additional minipreps of the same 6 parts. Digestion and ligation to obtain : RBS ( strong and weak ) + GFP, RBS + YFP. Culture of NM522 competent cells.





Tuesday


Transformation of the ligations into NM522 using a CaCl2 chemical transformation.





Wednesday


Selection of individual transformed colonies and start of solid and liquid culture.


We shoot our "Cobalt Buster's Clip" which introduces, in a funny way, our team : students, instructors and advisors. We dedicate the all afternoon to this shooting.





Thursday


Extraction, digestion and electrophoresis of the selected transformed cultures of individual colonies : the transformation had failed.





Friday