Team:Lyon-INSA-ENS/Realisation/Week5

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Revision as of 13:46, 25 July 2011




Week 5


From Monday the 4th of July to Friday the 8th of July 2011







Monday

Additional minipreps of the same 6 parts. Digestion and ligation to obtain : RBS ( strong and weak ) + GFP, RBS + YFP. Culture of NM522 competent cells.



Tuesday

Transformation of the ligations into NM522 using a CaCl2 chemical transformation.



Wednesday

Selection of individual transformed colonies and start of solid and liquid culture.

We shoot our "Cobalt Buster's Clip" which introduces, in a funny way, our team : students, instructors and advisors. We dedicate the all afternoon to this shooting.



Thursday

Extraction, digestion and electrophoresis of the selected transformed cultures of individual colonies : the transformation had failed.



Friday