Team:Lyon-INSA-ENS/Realisation/Week2

From 2011.igem.org

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One transformed colony seemed correct, the others were probably satellite colonies without the plasmid.<br/>
One transformed colony seemed correct, the others were probably satellite colonies without the plasmid.<br/>
Start of 5mL liquid culture of the main colony. <br/>
Start of 5mL liquid culture of the main colony. <br/>
Plating of the satellite colonies to check the presence of the plasmid <br/>
Plating of the satellite colonies to check the presence of the plasmid <br/>
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4 more new clones have grown : start of 5mL liquid cultures on these clones.
4 more new clones have grown : start of 5mL liquid cultures on these clones.
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Miniprep on the four clones. Verification of plasmid by digestion (EcoRI, HindIII)
Miniprep on the four clones. Verification of plasmid by digestion (EcoRI, HindIII)
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PCR from genomic DNA from E.coli strain MC4100 for three genes : RcnR (cobalt receptor), CsgAB (curli operon, part 1) and CsgEFG (curli operon, part 2)
PCR from genomic DNA from E.coli strain MC4100 for three genes : RcnR (cobalt receptor), CsgAB (curli operon, part 1) and CsgEFG (curli operon, part 2)
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Revision as of 07:43, 28 July 2011










Week 2


From Monday the 20th of June to Friday the 24th of June 2011





Monday


One transformed colony seemed correct, the others were probably satellite colonies without the plasmid.
Start of 5mL liquid culture of the main colony.
Plating of the satellite colonies to check the presence of the plasmid





Tuesday


4 more new clones have grown : start of 5mL liquid cultures on these clones.





Wednesday


Miniprep on the four clones. Verification of plasmid by digestion (EcoRI, HindIII)





Thursday


PCR from genomic DNA from E.coli strain MC4100 for three genes : RcnR (cobalt receptor), CsgAB (curli operon, part 1) and CsgEFG (curli operon, part 2)





Friday