Team:Lyon-INSA-ENS/Realisation/Week18

From 2011.igem.org

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<h6 style="text-align :left"> Monday </h6> <HR>
<h6 style="text-align :left"> Monday </h6> <HR>
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<FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/>
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Extraction, EP Digestion and Gel electrophoresis of the NM522/p85 clones. They are all good.
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<h6 style="text-align :left"> Tuesday </h6> <HR>
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<FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/>
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Transformations : MC4100/pIG2+p150, MC4100/pIG6+p150.
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Some problems with the transformations were noticed (only few clones), caused probably by the fact that the TSS wasn't fresh, so a new solution has been made and used.
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<h6 style="text-align :left"> Wednesday </h6> <HR>
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<h6 style="text-align :left"> Thursday </h6> <HR>
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<h6 style="text-align :left"> Wednesday </h6> <HR>
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<FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/>
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Transformations results : no clones for MC4100/pIG2+p150, MC4100/pIG6+p150.
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Transformations: MC4100/p85, PHL1414+piG3.
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Revision as of 17:41, 27 October 2011









Week 18


From Monday the 17th of October to Sunday the 23rd of October 2011







Monday


Strain construction

Extraction, EP Digestion and Gel electrophoresis of the NM522/p85 clones. They are all good.



Tuesday


Strain construction

Transformations : MC4100/pIG2+p150, MC4100/pIG6+p150. Some problems with the transformations were noticed (only few clones), caused probably by the fact that the TSS wasn't fresh, so a new solution has been made and used.



Wednesday


Strain construction

Transformations results : no clones for MC4100/pIG2+p150, MC4100/pIG6+p150. Transformations: MC4100/p85, PHL1414+piG3.



Friday









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