"
Team:Lyon-INSA-ENS/Realisation/Week18
From 2011.igem.org
| (One intermediate revision not shown) | |||
| Line 39: | Line 39: | ||
<h6 style="text-align :left"> Monday </h6> <HR> | <h6 style="text-align :left"> Monday </h6> <HR> | ||
| - | + | <br/> | |
| + | <p style=" line-height : 1.5em"> | ||
| + | <FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/> | ||
| + | |||
| + | Extraction, EP Digestion and Gel electrophoresis of the NM522/p85 clones. They are all good. | ||
| + | |||
| + | |||
| + | <p/> | ||
<br/><br/> | <br/><br/> | ||
| Line 45: | Line 52: | ||
<h6 style="text-align :left"> Tuesday </h6> <HR> | <h6 style="text-align :left"> Tuesday </h6> <HR> | ||
| - | + | <br/> | |
| - | + | <p style=" line-height : 1.5em"> | |
| + | <FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/> | ||
| + | Transformations : MC4100/pIG2+p150, MC4100/pIG6+p150. | ||
| + | Some problems with the transformations were noticed (only few clones), caused probably by the fact that the TSS wasn't fresh, so a new solution has been made and used. | ||
| + | <p/> | ||
| - | + | <br/><br/> | |
| - | + | ||
| - | + | ||
| - | <h6 style="text-align :left"> | + | <h6 style="text-align :left"> Wednesday </h6> <HR> |
| - | + | <br/> | |
| + | <p style=" line-height : 1.5em"> | ||
| + | <FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/> | ||
| + | Transformations results : no clones for MC4100/pIG2+p150, MC4100/pIG6+p150.<br/> | ||
| + | Transformations: MC4100/p85, PHL1414+piG3. | ||
| + | <p/> | ||
<br/><br/> | <br/><br/> | ||
| - | |||
<h6 style="text-align :left"> Friday </h6> <HR> | <h6 style="text-align :left"> Friday </h6> <HR> | ||
| - | + | <br/> | |
| + | <p style=" line-height : 1.5em"> | ||
| + | <FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/> | ||
| + | A 5ml liquid cultured for 4 clones of PHL1414/pIG3 and 4 clones of MC4100/p85 is started.<br/> | ||
| + | Extraction and digestion by E and P. | ||
| + | The four clones of MC4100/p85 are good. | ||
| + | <p/> | ||
<br/><br/> | <br/><br/> | ||
| - | |||
| - | |||
Latest revision as of 18:20, 27 October 2011
Week 18
From Monday the 17th of October to Sunday the 23rd of October 2011
Monday
Strain construction
Extraction, EP Digestion and Gel electrophoresis of the NM522/p85 clones. They are all good.
Tuesday
Strain construction
Transformations : MC4100/pIG2+p150, MC4100/pIG6+p150.
Some problems with the transformations were noticed (only few clones), caused probably by the fact that the TSS wasn't fresh, so a new solution has been made and used.
Wednesday
Strain construction
Transformations results : no clones for MC4100/pIG2+p150, MC4100/pIG6+p150.
Transformations: MC4100/p85, PHL1414+piG3.
Friday
Strain construction
A 5ml liquid cultured for 4 clones of PHL1414/pIG3 and 4 clones of MC4100/p85 is started.
Extraction and digestion by E and P.
The four clones of MC4100/p85 are good.
