Team:Lyon-INSA-ENS/Realisation/Protocols

From 2011.igem.org

Revision as of 19:32, 7 September 2011 by Alex273 (Talk | contribs)










General culture conditions





Antibiotics were used at the following concentrations :
Ampicilin (Amp) : 100µg/mL
Chloramphenicol (Cm) : 20µg/mL
Spectinomycin (Spc) : 100µg/mL
Kanamycin (Kan) : 30µg/mL
The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution.

The composition of the different media we have used is the following :
LB :
M63 :
M63G :
LB/2 :











Choose one of our protocols to read its description





. Biofilm quantification





. CaCl2 chemical transformation





. Fermentas digestion





. Ligation





. NucleoSpin Plasmid QuickPure : Isolation of high-copy plasmid DNA from E. coli





. Ozyme digestion





. Plasmid or Cosmid DNA purification using HiSpeed Plasmid Midi kits





. Plasmid DNA purification using QIAprep® Spin Miniprep Kit





. Pouring of plates





. Storage of strains





. TSS chemical transformation




ENS assystem Biomérieux INSA INSA
Retrieved from "http://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Protocols"