Team:Lyon-INSA-ENS/Realisation/Protocols

From 2011.igem.org

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Spectinomycin (Spc) : 100µg/mL <br>
Spectinomycin (Spc) : 100µg/mL <br>
Kanamycin (Kan) : 30µg/mL<br>
Kanamycin (Kan) : 30µg/mL<br>
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The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution. <br><br>
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The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution. <br><br></p>
<p style=" line-height : 1.5em"><b>The composition of the different media we have used is the following :</b><br><br>
<p style=" line-height : 1.5em"><b>The composition of the different media we have used is the following :</b><br><br>

Revision as of 10:09, 13 September 2011






General culture conditions






Antibiotics were used at the following concentrations :

Ampicilin (Amp) : 100µg/mL
Chloramphenicol (Cm) : 20µg/mL
Spectinomycin (Spc) : 100µg/mL
Kanamycin (Kan) : 30µg/mL
The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution.

The composition of the different media we have used is the following :

LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.
M63G : 100/1/1 (v/v) of M63, glucose 20% and LB
LB/2 : 50/50 (v/v) mix of LB and water








ENS assystem Biomérieux INSA INSA