Team:Lyon-INSA-ENS/Project/Achievement

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         <img src="https://static.igem.org/mediawiki/2011/0/0e/Drapeau_francais.jpg"; width=20px; /> <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Project/AchievementFr">Version Francaise</a>
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         <img src="https://static.igem.org/mediawiki/2011/0/0e/Drapeau_francais.jpg"; width=20px; /> <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Project/AchievementFr">Version Fran&ccedil;aise</a>
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<h1><font color="green"> A race between two different strategies was used to obtain the first part </font></h1> <HR>
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              Achievements<br><HR>
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              <br/>
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In E. coli the curli-producing system is organized in two divergeant operons with the genes
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csgA, csgB and csgC on one side and csgD, csgE, csgF and csgG of the other one. So to
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achieve the first approach of the project, it is necessary to begin by the creation of three "sub-
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parts": the first one would be the part with the sole promoter PrcnA, the second would be the
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part with the genes csgA and csgB and final one would be the part with the genes csgE, csgF
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and csgG.
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<li>We lived a <b>great human experience</b>, met new friends and <b>had fun</b>!!</li><br><br>
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The synthesis of this first DNA part (the cobalt-inducible promoter Prcn-csgBAEFG, which is
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<li>We acquired <b>new knowledge and competencies</b> dealing with scientific and logistic issues.</li><br><br>
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designed to induce formation of a biofilm and to promote cell adhesion) was conceived as as a
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<li>We submitted <b> <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Project/Parts">2 documented parts</a></b> to the registry and showed that they work as expected.</li><br><br>
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competition between two methods: a completely synthetic approach, and a "manual" method
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<li>We characterized a <b><a href="http://partsregistry.org/Part:BBa_K342003">pre-existing part</a></b>.</li><br><br>
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involving PCR steps followed by ligations. <br/>
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<li>We <b><a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Sponsors/Collaboration">collaborated</a></b> with another iGEM team : TU Delft.</li><br><br>
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<li><p style="line-height:1.5em;text-indent:0%;">
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We <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Communication/Main">presented our project and Synthetic Biology</a> in many ways :  in different <b>websites</b>,<br/> in <b>newspapers</b>, on the <b>radio</b>, on <b>TV</b>, … and even <b>in music</b> ; in order to inform a public <br/>as varied as possible.
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<li>We <b>get our project close to reality</b> meeting experts to consider <b> <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Project/Industrialization">industrialization</a></b>.</li><br/><br/>
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<li>We explored <b><a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Safety/Intro"> safety implications </a></b> of the project as a whole.</li><br/>
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              <li style="line-height : 1.5em"> The first approach consist in ordering the whole part at a private company (Genecust).</li>
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              <li style="line-height : 1.5em"> The second approach was to made it directly at the bench, this approach included three
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steps: first the amplification by PCR of each of the sub parts, second a mutagenesis
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step to remove all the natural internal EcoRI sites located in the sub parts, and finally
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the ligation of these parts.</li>
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Both approches were initiated at the same time, and if the second one allowed us to obtain
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PCR amplifications of the correct size (see figure) and a complete Prcn-csgBAEFG
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construction, unfortunately sequencing analysis revealed unexpected mutations that were not
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removed before reception of the whole part made by Genecust. In brief the "click and order"
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strategy wins the race !
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Latest revision as of 23:33, 21 September 2011









Achievements



  • We lived a great human experience, met new friends and had fun!!


  • We acquired new knowledge and competencies dealing with scientific and logistic issues.


  • We submitted 2 documented parts to the registry and showed that they work as expected.


  • We characterized a pre-existing part.


  • We collaborated with another iGEM team : TU Delft.


  • We presented our project and Synthetic Biology in many ways : in different websites,
    in newspapers, on the radio, on TV, … and even in music ; in order to inform a public
    as varied as possible.



  • We get our project close to reality meeting experts to consider industrialization.


  • We explored safety implications of the project as a whole.

















ENS assystem Biomérieux INSA INSA
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