"
Promoters and Terminators
The iGEM registry is lacking in promoter and terminators sequences for yeast, especially when compared with the number available for use in E. coli. In order to address this problem and increase the use of yeast in synthetic biology we have decided to provide a library of promoter and terminator sequences from 12 yeast genes: 4 causing high expression, 4 causing medium expression, and 4 causing low expression. We hope these BioBrick compatible parts will make it easier for future iGEM teams to work with Saccharomyces cerevisiae.
Promoters
Promoter sequences that have been BioBricked:
| RPS8Bp | BAP2p | FCY2p | Gal 1/10p | HHO1p |
|---|---|---|---|---|
| KRE9p | PRY1p | STM1p | TDH3p | RPS2p |
Terminators
3'UTR sequences that have been BioBricked:
| ARD1utr | BAP2utr | FCY2utr | HHo1utr | KRE9utr |
|---|---|---|---|---|
| PRY1utr | RPL8Autr | RPL24Autr | RPS2utr | RPS8Butr |
| TDH3utr |
Promoters and 3'UTRs for Golden Gate Assembly
We also have a second promoter/3'UTR library consisting of the same sequences with custom overhangs with BsaI sites rather than the BioBrick sites. These are being used in our two violacein projects, where they will allow Golden Gate assembly of violacein expression cassettes.
