Team:Johns Hopkins/Data

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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530027 VTC4] Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of L-Galactose to L-Galactono-1,4-Lactone.
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530027 VTC4] Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of L-Galactose to L-Galactono-1,4-Lactone.
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===Promoters===
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530003 KRE9p] KRE9 Promoter is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530008 TDH3p] TDH3 Promoter is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
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===Terminators===
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530019 HHO1utr] HHO1 Terminator is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530020 ARD1utr] ARD1 Terminator is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
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===Vectors===
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530032 pRSBB413 HIS3 CEN/ARS Yeast Shuttle Vector] Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and HIS3 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector.
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530039 pRSBB426 URA3 2Micron Episomal Yeast Shuttle Vector] Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and URA3 selectable marker for S. cerevisiae. Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.
=== Existing Parts Characterized ===
=== Existing Parts Characterized ===

Revision as of 03:17, 28 September 2011

VitaYeast - Johns Hopkins University, iGEM 2011

Data Page
Vitamin A Production
Beta-carotene datapage.jpg
Vitamin C Production
L-ascorbate datapage.jpg
Yeast Toolkit
Toolkit datapage.jpg
Pros.jpg
Utrs.jpg
Vectors.jpg

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530004 STM1p]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530005 BAP2p]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530007 PRY1p]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530006 BAP2u]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530014 KRE9u]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530017 FCY2u]

Integrating vectors
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530028 pRSBB403 (HIS3)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530029 pRSBB404 (TRP1)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530030 pRSBB405 (LEU2)]

Favorite New Parts

Vitamin A Production

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530000 CrtYB] Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is a fusion, it is the full Phytoene Synthase enzyme spliced with a Lycopene B-Cyclase enzymatic domain. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.
  2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530001 CrtE] Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.
  3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530002 CrtI] Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Phytoene Desaturase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes the conversion of Phytoene to Lycopene.

Vitamin C Production

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530025 VTC2] Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of GDP-L-Galactose to L-Galactose-1-P.
  2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530026 GME] Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of L-Galactose-1-P to L-Galactose.
  3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530027 VTC4] Enzyme in the pathway required for L-Ascorbate Acid Synthesis. This sequence was taken from plants. It catalyzes the conversion of L-Galactose to L-Galactono-1,4-Lactone.


Promoters

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530003 KRE9p] KRE9 Promoter is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
  2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530008 TDH3p] TDH3 Promoter is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.

Terminators

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530019 HHO1utr] HHO1 Terminator is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
  2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530020 ARD1utr] ARD1 Terminator is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.

Vectors

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530032 pRSBB413 HIS3 CEN/ARS Yeast Shuttle Vector] Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and HIS3 selectable marker for S. cerevisiae. Fully compatible for use with Standard Assembly. Contains EcoRI, XbaI, SpeI, and PstI in the multiple cloning site, in that order. This vector is a CEN/ARS episomal yeast vector.
  2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530039 pRSBB426 URA3 2Micron Episomal Yeast Shuttle Vector] Yeast shuttle vector for propagation in E. coli and transformation into S. cerevisiae. Contains an Ampicillin resistance marker for E. coli and URA3 selectable marker for S. cerevisiae. Contains EcoRI, NheI, SpeI, and PstI in the multiple cloning site, in that order. For assembly, this vector differs from a Standard Assembly vector by replacing XbaI with NheI. This vector is a 2micron episomal yeast vector.

Existing Parts Characterized

  1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_J63006 Gal1p]



All Submitted Parts
<groupparts>iGEM011 Johns_Hopkins</groupparts>



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