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Team:Imperial College London/Notebook/week5
From 2011.igem.org
Diary
This is our diary page which records the daily activities of the team. Click on the links below to see a summary of events and activities happening each week.
Contents |
Week 13: 1st August to 7th August
Monday, 1st August
Seeing the month change from July to August has made us realize how much time has passed. It has been four weeks since we started this endeavour and we are still nowhere near where we want to be. Time is ticking away, as cliché as that may sound.
The day started off with the absence of two of our members that had fallen ill over the weekend. We missed them dearly but had no time to stop working! Nic has booked a session on Thursday to use the wide-field microscope. Operation fancy GFP chemotaxis stuff is a go. On the Arabidopsis team, Ming did what he does best (in the lab that is).
Nina and Si continued to plow through the modelling of chemotaxis. Si has also arranged a meeting with a PhD student on Thursday to discuss modelling root architecture. Finally Nikki and Yuanwei put their heads together for the good of the wiki.
Tuesday, 2nd August
Today was a good day. Chris and Nikki had a succesfull restriction digest of the backbone vectors. Chris got to do Nick's dirty work while he was in Silwood with Rebekka talking to ecology expert Alex Milku. When Nick got back he had the unfortunate finding that the bacteria he was going to use done have flagella, but its all good in tha hood because James found some GFP bacteria that move!
The modellers are in their dungeon modelling. Wiki man is making great progress.
Ming loves his Arabidopsis so were all happy they are being loved.
Wednesday, 3rd August
Today we had the pleasure of welcoming Kieran, an A-level student on a work experience placement. Frank spent the morning showing him around the labs and showing him the mystical ways of making broth. We gave him some time to get acquainted with our project before he helped Ming with preparing a whole new batch of plant media for the experiments and the growing plants.
We had another visit from the RCA guys, Koby, CJ and Pei, which was cool. We had a cool discussion about some of our human practices work as well as the more artistic side of things such as the wiki and videos.
Nick had a stroke of bad luck this morning, as it seemed that the strain of bacteria he was using for his transformation appeared to have lost their antibiotic resistance genes. Oh well.
The modelling team of Si and Nina were on top form as usual. Si finished a cool animation and provided Nick with the necessary numbers for his experiments. Nina spent some time working on the most efficient way to deliver auxin to the roots, which we should probably have a group discussion about.
The day ended with Chris sorting out the chemicals for his auxin assays and a pair of transformations from Frank and Nick. Frank transformed some E.coli with Berkeley 08's killswitch module and Nick transformed E.coli K12 BL21DE3 with a plasmid affectionately referred to as pRhys, after its supplier.
Thursday, 4th August
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Friday, 5th August
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