Team:Harvard/Technology

From 2011.igem.org

(Difference between revisions)
Line 18: Line 18:
=Chip-Based Synthesis=
=Chip-Based Synthesis=
 +
See our [https://2011.igem.org/Team:Harvard/Project/Synthesize Synthesize] page for details on how we applied chip synthesis to zinc finger proteins.
 +
Using microchip synthesis (provided by Agilent Technologies), we have 55,000 potential zinc fingers (whose sequences were generated by Team Harvard's [[Team:Harvard/Project/Design|bioinformatics]]) to test. These fingers will then be tried against the DNA sequences we wish to bind.  
Using microchip synthesis (provided by Agilent Technologies), we have 55,000 potential zinc fingers (whose sequences were generated by Team Harvard's [[Team:Harvard/Project/Design|bioinformatics]]) to test. These fingers will then be tried against the DNA sequences we wish to bind.  
*Original Paper: http://www.nature.com/nbt/journal/v28/n12/full/nbt.1716.html
*Original Paper: http://www.nature.com/nbt/journal/v28/n12/full/nbt.1716.html
Line 23: Line 25:
=MAGE=
=MAGE=
 +
See our [https://2011.igem.org/Team:Harvard/Project/Test#Building_the_selection_strain:_MAGE Test] page for details on how we used MAGE to build our selection strain.
 +
Multiplex automated genome engineering (MAGE) is a new method for large-scale programming and evolution of cells. MAGE simultaneously targets many locations on the chromosome, thus producing combinatorial genomic diversity.  
Multiplex automated genome engineering (MAGE) is a new method for large-scale programming and evolution of cells. MAGE simultaneously targets many locations on the chromosome, thus producing combinatorial genomic diversity.  
*Original Paper: http://www.nature.com/nature/journal/v460/n7257/full/nature08187.html
*Original Paper: http://www.nature.com/nature/journal/v460/n7257/full/nature08187.html
Line 28: Line 32:
=Lambda Red Mediated Recombineering=
=Lambda Red Mediated Recombineering=
 +
See our [https://2011.igem.org/Team:Harvard/Project/Test#Building_the_selection_strain:_Lambda_Red_Recombineering Test] page for details on how we used lambda red to build our selection strain.
 +
Genes can be altered by recombination with linear DNA molecules. This requires a high internal DNA concentration, achievable by electroporation. The lambda red system allows efficient recombination between homologous sequences as short as 40 bp, which frees us of the need to provide long tracts of homology for recombination into the chromosome.
Genes can be altered by recombination with linear DNA molecules. This requires a high internal DNA concentration, achievable by electroporation. The lambda red system allows efficient recombination between homologous sequences as short as 40 bp, which frees us of the need to provide long tracts of homology for recombination into the chromosome.
*Gene Knockouts and Exchanges by Linear Transformation: http://rothlab.ucdavis.edu/protocols/Lin.Transform.html
*Gene Knockouts and Exchanges by Linear Transformation: http://rothlab.ucdavis.edu/protocols/Lin.Transform.html

Revision as of 00:53, 22 October 2011

bar

Our project uses 3 new technologies: chip-based synthesis of DNA[1], multiplex automated genome engineering (MAGE)[2][3], and lamba red recombination[4][5], along with more traditional bioinformatics.

Although the project is the first to utilize several key technologies in novel ways, these technologies were developed outside of Harvard iGEM. The multiplex automated genome engineering (MAGE) method was developed by Harris Wang et al[2]. The chip-based synthesis method that we used to synthesize oligos for our zinc finger pool was developed by Sri Kosuri et al[1], and the actual oligo synthesis was generously provided by Agilent Technologies, a sponsor of iGEM. Lambda red was originally developed by Yu et al[4].

We hope that future iGEM teams will also use these techniques in their own synthetic biology projects.

Bioinformatics

See our Design page for details on the computational aspects of our project technology.

Zinc Finger Binding Site Finder

Check out our Zinc Finger Binding Site Finder Tool. This tool was designed and used to search the human genome for the six target DNA sequences that we used to design our custom zinc finger arrays.

Chip-Based Synthesis

See our Synthesize page for details on how we applied chip synthesis to zinc finger proteins.

Using microchip synthesis (provided by Agilent Technologies), we have 55,000 potential zinc fingers (whose sequences were generated by Team Harvard's bioinformatics) to test. These fingers will then be tried against the DNA sequences we wish to bind.

MAGE

See our Test page for details on how we used MAGE to build our selection strain.

Multiplex automated genome engineering (MAGE) is a new method for large-scale programming and evolution of cells. MAGE simultaneously targets many locations on the chromosome, thus producing combinatorial genomic diversity.

Lambda Red Mediated Recombineering

See our Test page for details on how we used lambda red to build our selection strain.

Genes can be altered by recombination with linear DNA molecules. This requires a high internal DNA concentration, achievable by electroporation. The lambda red system allows efficient recombination between homologous sequences as short as 40 bp, which frees us of the need to provide long tracts of homology for recombination into the chromosome.

  • Gene Knockouts and Exchanges by Linear Transformation: http://rothlab.ucdavis.edu/protocols/Lin.Transform.html
  • Open Wet Ware Protocol: http://openwetware.org/wiki/Recombineering/Lambda_red-mediated_gene_replacement
  • Our Protocol: https://2011.igem.org/Team:Harvard/Protocols#Lambda_Red

Gibson (Isothermal) Assembly

An isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5′ exonuclease, a DNA polymerase and a DNA ligase.

References

1. Sriram Kosuri, Nikolai Eroshenko, Emily M LeProust, Michael Super, Jeffrey Way, Jin Billy Li, George M Church. (2010). Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips. Nature Biotechnology, 28(12):1295-9. [http://www.nature.com/nbt/journal/v28/n12/full/nbt.1716.html]

2. Harris H. Wang, Farren J. Isaacs, Peter A. Carr, Zachary Z. Sun, George Xu, Craig R. Forest, George M. Church. Programming cells by multiplex genome engineering and accelerated evolution. (2009). Nature, 460(7257):894-8. [http://www.nature.com/nature/journal/v460/n7257/full/nature08187.html]

3. Isaacs FJ, Carr PA, Wang HH, Lajoie MJ, Sterling B, Kraal L, Tolonen AC, Gianoulis TA, Goodman DB, Reppas NB, Emig CJ, Bang D, Hwang SJ, Jewett MC, Jacobson JM, Church GM. (2011). Precise manipulation of chromosomes in vivo enables genome-wide codon replacement. Science, 333(6040):348-53. [http://www.sciencemag.org/content/333/6040/348.full]

4. Yu D., H. M. Ellis, et al. (2000). An efficient recombination system for chromosome engineering in Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 97(11): 5978-5983.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC165854/]

5. Mosberg JA, Lajoie MJ, Church GM. Lambda red recombineering in Escherichia coli occurs through a fully single-stranded intermediate. Genetics 2010;186:791-799.[http://www.genetics.org/content/186/3/791]