Team:Harvard/Judging

From 2011.igem.org

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=Accomplishments=
=Accomplishments=
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'''✓''' Researched zinc finger proteins and choose 6 novel, clinically relevant target sequences <br>
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'''✓''' Researched zinc finger proteins and choose 6 novel, <div style="color:#C22227">clinically relevant target sequences </div><br>
'''✓''' Generated 55,000 zinc finger protein sequences using bioinformatics <br>
'''✓''' Generated 55,000 zinc finger protein sequences using bioinformatics <br>
'''✓''' Expressed those 55,000 sequences in E.coli, with a sub-library for each target <br>
'''✓''' Expressed those 55,000 sequences in E.coli, with a sub-library for each target <br>
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'''✓''' Researched intellectual property and how it applies to zinc finger proteins<br>
'''✓''' Researched intellectual property and how it applies to zinc finger proteins<br>
'''✓''' Made protocols, Biobricks, and source code freely available, so that others can adapt them for other projects <br>
'''✓''' Made protocols, Biobricks, and source code freely available, so that others can adapt them for other projects <br>
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Revision as of 15:40, 28 October 2011

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Wiki Highlights

  • Read our project description and summary on our Project page: more details on how team members completed work for the three sections of our project are on Design, Synthesize, and Test pages, respectively.
  • Please be sure to stop by our poster during the poster session with any questions.

Accomplishments

Researched zinc finger proteins and choose 6 novel,
clinically relevant target sequences

Generated 55,000 zinc finger protein sequences using bioinformatics
Expressed those 55,000 sequences in E.coli, with a sub-library for each target
Created a genomic one-hybrid selection system sensitive enough to detect one in a million hits
Found up to 15 novel zinc finger proteins
Researched intellectual property and how it applies to zinc finger proteins
Made protocols, Biobricks, and source code freely available, so that others can adapt them for other projects