Team:Fatih Turkey/Results


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1. technique; in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization.

2. Alternative cure for septic shock; after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.

3. Possible new structure of antibiotic-like drug; antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections.

4. Possibility of producing longtime sterile layers; if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In case of a possible infection on surface, LALF will stop bacteria growth.

5. Alternative cure method for bacterial diseases; diseases like urinary system infection or gastritis are caused by gram negative bacteria. In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.

On the other hand, our secondary protein, reflectin, can be used in varying applications.

1. Camouflage material; this protein allows its host, cephalopod, to hide when cephalopod hunts or to defend itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans.

2. New material of dye for varying applications; Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.

3. Possibility of producing specialized light colors; in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.

For our project lab executions;

1. LALF based anti-LPS factor system has been worked expectedly

2.we have constructed and submitted 12 biobrick. Three devices working in two for B.subtilis one for E.coli work expectedly.

3. Our B.subtilis specific promoter with SacB- extracellur signal- has been seen working well.

4. We have synthesized Reflectin 1a protein in E.coli; however, because we did not have enough time to purify it to visualize via aid-eye, we could not use Reflectin 1a device as biosensor for our lalf-LPS interaction in B.subtilis- E.coli mixed culture.

For Human Practice; We have performed the activities as follows;

1. Providing Sporocide Method for prevention from spores of B.subtilis in further B.subtilis synthetic biology project.

2. On the other hand, our secondary protein, reflectin, can be used in varying applications.

3. Preparing a novel board game `Canvas Time` to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition. .

4.Performing open-lab to public meetings such as gifted children studying in several schools of Ankara, undergraduate students from Hacettepe University Medical School and postgraduate students from Ankara University Medical School

5.Kindergarten synthetic biology trip to teach synthetic biology to five-year-old children.