Team:Edinburgh/Data

From 2011.igem.org

(Difference between revisions)
Line 13: Line 13:
==Cell Surface Display System==
==Cell Surface Display System==
-
[[Image:Edinburgh-Data-Cell-Display.png|thumb|center|732px|The completed system should contain:<br>
+
[[Image:Edinburgh-Data-Cell-Display.png|thumb|center|710px|The completed system should contain:<br>
&nbsp; A '''promoter''' (<partinfo>BBa_K523000</partinfo>) controlling:<br>
&nbsp; A '''promoter''' (<partinfo>BBa_K523000</partinfo>) controlling:<br>
&nbsp; &nbsp; an '''INP&mdash;Endoglucanase''' fusion (<partinfo>BBa_K523008</partinfo> + <partinfo>BBa_K523011</partinfo>)<br>
&nbsp; &nbsp; an '''INP&mdash;Endoglucanase''' fusion (<partinfo>BBa_K523008</partinfo> + <partinfo>BBa_K523011</partinfo>)<br>

Revision as of 19:32, 2 September 2011

Data Page

The iGEM rules require us to have simple illustrations of how our devices work and where the Parts function in the system; and links to the Registry for the parts/constructs for which we have produced data.

See the sample Data page.

Cell Surface Display System

The completed system should contain:
  A promoter (<partinfo>BBa_K523000</partinfo>) controlling:
    an INP—Endoglucanase fusion (<partinfo>BBa_K523008</partinfo> + <partinfo>BBa_K523011</partinfo>)
    an INP—β-glucosidase fusion (<partinfo>BBa_K523008</partinfo> + <partinfo>BBa_K523010</partinfo>)
    an INP—Exoglucanase fusion (<partinfo>BBa_K523008</partinfo> + <partinfo>BBa_K523009</partinfo>)
  Ribosome Binding Sites are indicated as green ovals.

Cellulose degradation is shown at top. In reality, tens of thousands of enzymes will cover the outer membrane in random places.

A test system to prove that <partinfo>BBa_K523008</partinfo> can be used to carry proteins to the outer membrane uses a fusion to Yellow Fluorescent Protein (YFP) instead.

Phage Display System

To do