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Team:Cornell/Week 15
From 2011.igem.org
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==Thursday, September 15== | ==Thursday, September 15== | ||
| + | Afternoon lab work done by: Youjin Cho, Charlie Chung | ||
| + | :*Gel purification of digested light-lysis gene insert | ||
| + | :*Ligation of light-lysis gene insert with pSB1C3 iGEM backbone | ||
| + | |||
| + | Afternoon lab work done by: Maneesh Gupta | ||
| + | :*Tested effect of fluorescein with lysate on streptavidin-biotin binding to see if lysate interferes with their affinity | ||
| + | ::- Used 5:1 ratio of lysate to fluorescein | ||
| + | ::- Chips used in the experiment were coated yesterday (streptavidin is 1 day old) | ||
| + | ::- Flow rate = 1µL/min | ||
==Friday, September 16== | ==Friday, September 16== | ||
==Saturday, September 17== | ==Saturday, September 17== | ||
Revision as of 19:18, 15 September 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
September 11th - September 17th
Sunday, September 11
~God bless America -- never forget~
Monday, September 12
Afternoon lab work done by: Nancy Li, Charlie Chung
- Prepared ten 3mL LB cultures (with 3µL carbenicillin) of colonies picked from plates dated Sept 3
- - RFP + Avi-Tagged pZE12 in DH5α
- - vioA + Avi-Tagged pZE12 in DH5α
- - vioB + Avi-Tagged pZE12 in DH5α
- - vioE + Avi-Tagged pZE12 in DH5α
- - RFP + Avi-Tagged pZE12 in MC4100
- - vioA + Avi-Tagged pZE12 in MC4100
- - vioB + Avi-Tagged pZE12 in MC4100
- - vioE + Avi-Tagged pZE12 in MC4100
- - Control tube is just a colony with pZE12 (no insert gene)
- - LB with carbenicillin (no inoculation with bacteria)
- Incubating at 37°C in the shaker at Weill Hall
- Cultures are to be lysed for release of natively biotinylated RFP and vio enzymes in characterizing streptavidin-coated microfluidics chips
- Did not extensively search for L-tryptophan (starting substrate in the synthesis of prodeoxyviolacein) in the reagents shelf of our Weill Lab space, but does not appear to be in plain view (may be tucked away in an obscure corner?)
Tuesday, September 13
Afternoon lab work done by: Charlie Chung
- Objective: Transfer yesterday's 3mL starter culture into today's larger scale culture (20mL LB + 20µL ampicillin)
- Autoclaved two 1L LB at 1:30pm (~1.5 hour cycle)
- Claire's batch of five autoclaved 250mL flasks are ready for use
- Prepared stock solution of 1000x ampicillin (100mg/mL)
- - Weigh out 1g ampicillin sodium salt (Sigma)
- - Dissolve in 10mL ddH2O
- - Syringe filter (0.22µm membrane) the ampicillin solution
- Stored in the bottom shelf of Weill Lab refrigerator (it's in a 15mL conical -- will later aliquot into Eppendorfs)
Evening lab work done by: Claire Paduano
- Prepared cultures of Avi-Tagged vioA, B, E and RFP in 20mL LB with 20uL ampicillin
Wednesday, September 14
Thursday, September 15
Afternoon lab work done by: Youjin Cho, Charlie Chung
- Gel purification of digested light-lysis gene insert
- Ligation of light-lysis gene insert with pSB1C3 iGEM backbone
Afternoon lab work done by: Maneesh Gupta
- Tested effect of fluorescein with lysate on streptavidin-biotin binding to see if lysate interferes with their affinity
- - Used 5:1 ratio of lysate to fluorescein
- - Chips used in the experiment were coated yesterday (streptavidin is 1 day old)
- - Flow rate = 1µL/min
