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Team:Cornell/Week 12
From 2011.igem.org
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August 21st - August 27th
Sunday, August 21
Evening lab work done by: Youjin Cho, Charlie Chung
- Picking Colonies and Starting Up Cultures of vioB Ligation Reaction
- (Avi-Tagged pZE12 backbone) control plate shows no growth of colonies
- (vioB + Avi-Tagged pZE12 backbone) plate shows numerous colonies
- - Picked three colonies (vioB+Avi+BB #1, 2, 3) and started 3mL cultures in LB with 3μL ampicillin
- - Incubated overnight in the 37°C shaker of Room 304
- PCR Reaction Setup of GFP (because sequencing results were negative)
- 38.5μL ddH2O
- 5μL 10x buffer
- 2.5μL dNTP
- 1μL forward GFP primer
- 1μL reverse GFP primer
- 1μL (pZE12+GFP) backbone template
- 1μL Vent DNA polymerase
- 50μL Total
- Annealing temp 54.2
1min elongation
