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Team:Cornell/Week 12
From 2011.igem.org
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:*(Avi-Tagged pZE12 backbone) control plate shows no growth of colonies | :*(Avi-Tagged pZE12 backbone) control plate shows no growth of colonies | ||
:*(vioB + Avi-Tagged pZE12 backbone) plate shows numerous colonies | :*(vioB + Avi-Tagged pZE12 backbone) plate shows numerous colonies | ||
| - | + | ::- Picked three colonies (vioB+Avi+BB #1, 2, 3) and started 3mL cultures in LB with 3μL ampicillin | |
| - | + | ::- Incubated overnight in the 37°C shaker of Room 304 | |
| - | :'''PCR Reaction Setup of GFP'''(''because sequencing results were negative'') | + | :'''PCR Reaction Setup of GFP''' (''because sequencing results were negative'') |
| + | :::38.5μL ddH2O | ||
| + | :::5μL 10x buffer | ||
| + | :::2.5μL dNTP | ||
| + | :::1μL forward GFP primer | ||
| + | :::1μL reverse GFP primer | ||
| + | :::1μL (pZE12+GFP) backbone template | ||
| + | :::<u>1μL Vent DNA polymerase</u> | ||
| + | :::50μL Total | ||
| + | ::*Annealing temp 54.2 | ||
| + | 1min elongation | ||
==Monday== | ==Monday== | ||
Revision as of 23:16, 21 August 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
August 21st - August 27th
Sunday, August 21
Evening lab work done by: Youjin Cho, Charlie Chung
- Picking Colonies and Starting Up Cultures of vioB Ligation Reaction
- (Avi-Tagged pZE12 backbone) control plate shows no growth of colonies
- (vioB + Avi-Tagged pZE12 backbone) plate shows numerous colonies
- - Picked three colonies (vioB+Avi+BB #1, 2, 3) and started 3mL cultures in LB with 3μL ampicillin
- - Incubated overnight in the 37°C shaker of Room 304
- PCR Reaction Setup of GFP (because sequencing results were negative)
- 38.5μL ddH2O
- 5μL 10x buffer
- 2.5μL dNTP
- 1μL forward GFP primer
- 1μL reverse GFP primer
- 1μL (pZE12+GFP) backbone template
- 1μL Vent DNA polymerase
- 50μL Total
- Annealing temp 54.2
1min elongation
