June 26
Start overnight cultures of T7 polymerase (K145001), mCherry (J06702), Lac Promoter (R0010), double terminator (B0014 and B0015) and Tet Promoter (R0040)
Transfer 0.5 mL aliquots of BPA and 5 mL aliquots 17a-ethynylestradiol cultures from June 23 to fresh minimal media
This Week
Ethanol precipitation of DNA extractions from LA river samples
Miniprep of T7 polymerase (K145001), mCherry (J06702), Lac Promoter (R0010), double terminator (B0014 and B0015) and Tet Promoter (R0040) and submit for sequencing
PCR of parts for pNT001 (Test plasmid for K123000)
Purification/Gibson assembly for pNT001
Cutting LA River DNA for insertion into fosmids
Email other teams
Insert LA River DNA into fosmids
Transform fosmids into E. coli and start enrichment cultures
Continue gene fishing
Send off continued forward sequencing for HER
PCR of parts for pNT002 and assembly
Use pNT001 and pNT002 to test degradation parts
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