Team:Caltech/Week 3

From 2011.igem.org

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Streak out cells from fosmid kit<br/>
Streak out cells from fosmid kit<br/>
Prepare antibiotic stocks (Ampicillin, Chloramphenicol)<br/><br/>
Prepare antibiotic stocks (Ampicillin, Chloramphenicol)<br/><br/>
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Miniprep Results <br/>
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===Results===
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Miniprep <br/>
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Revision as of 02:46, 28 June 2011


Caltech iGEM 2011



Home

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References

Support

June 26

Start overnight cultures of T7 polymerase (K145001), mCherry (J06702), Lac Promoter (R0010), double terminator (B0014 and B0015) and Tet Promoter (R0040)

June 27

Miniprep of T7 polymerase (K145001), mCherry (J06702), Lac Promoter (R0010), double terminator (B0014 and B0015) and Tet Promoter (R0040) and submit for sequencing
Transfer 0.5 mL aliquots of BPA and 5 mL aliquots 17a-ethynylestradiol cultures from June 23 to fresh minimal media
Checked DDT and nonylphenol cultures, will wait until tomorrow to transfer
Ethanol precipitation of DNA extractions from LA river samples following protocol
Streak out cells from fosmid kit
Prepare antibiotic stocks (Ampicillin, Chloramphenicol)

Results

Miniprep

Part Concentration(ng/ul)
B0014 230.5
B0015 254.1
J06702 301.3
K145001 205.6
R0010 117.3
R0040 156.8

We found growth in one of our BPA enrichment cultures, and slight growth in our ethinyl estradiol cultures. We transferred these vials today.

June 28

Send off continued forward sequencing for HER
Prepare gel of control fosmid DNA to practice gel extraction technique
PCR for Gibson
Gibson assembly of pNT001 and pNT002

This Week

PCR of parts for pNT001 (Test plasmid for K123000)
Purification/Gibson assembly for pNT001
Email other teams
PCR of parts for pNT002 and assembly
Use pNT001 and pNT002 to test degradation parts


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