Team:Caltech/Week 3
From 2011.igem.org
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Ethanol precipitation of DNA extractions from LA river samples following protocol<br/> | Ethanol precipitation of DNA extractions from LA river samples following protocol<br/> | ||
Streak out cells from fosmid kit<br/> | Streak out cells from fosmid kit<br/> | ||
- | Prepare antibiotic stocks (Ampicillin, Chloramphenicol)</p> | + | Prepare antibiotic stocks (Ampicillin, Chloramphenicol)<br/><br/> |
+ | Miniprep Results <br/> | ||
+ | <table border="1"> | ||
+ | <tr> | ||
+ | <th>Part</th> | ||
+ | <th>Concentration(ng/ul)</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>B0014</td> | ||
+ | <td>230.5</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>B0015</td> | ||
+ | <td>254.1</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>J06702</td> | ||
+ | <td>301.3</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>K145001</td> | ||
+ | <td>205.6</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>R0010</td> | ||
+ | <td>117.3</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>R0040</td> | ||
+ | <td>156.8</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | We found growth in one of our BPA enrichment cultures, and slight growth in our ethinyl estradiol cultures. We transferred these vials today. | ||
+ | </p> | ||
==June 28== | ==June 28== |
Revision as of 02:42, 28 June 2011
Project |
June 26Start overnight cultures of T7 polymerase (K145001), mCherry (J06702), Lac Promoter (R0010), double terminator (B0014 and B0015) and Tet Promoter (R0040) June 27Miniprep of T7 polymerase (K145001), mCherry (J06702), Lac Promoter (R0010), double terminator (B0014 and B0015) and Tet Promoter (R0040) and submit for sequencing
We found growth in one of our BPA enrichment cultures, and slight growth in our ethinyl estradiol cultures. We transferred these vials today. June 28Send off continued forward sequencing for HER This WeekPCR of parts for pNT001 (Test plasmid for K123000)
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