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Revision as of 07:48, 28 October 2011

Immobilization and recrystallisation of S-layer proteins

You have your S-layer protein monomer solution? Then you are at most five hours away from your nanobiotechnological device - and this only because you have to wait 4 hours for the recrystallization and immobilization of your S-layer proteins. But before you begin...

Choose your surface

Silicon dioxide beads.

A silicon waver.

S-layers can reassemble on a lot of different surfaces like gold, nylon, functionalized glass or silicon dioxide. We used silicon dioxide because it is cheap and easy to handle. For microscopy we used silicon wavers with a silicon dioxide surface. Then...

Let the immobilization begin

A vertical rotator which we used for coating silica beads with S-layers.

HBSS and recrystallization buffer for recrystallization.