Team:Bielefeld-Germany/Data Page

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Revision as of 13:29, 17 September 2011 by Manuelw (Talk | contribs)

How our System works

Bielefeld 2011 S-Layer-Produktion lysis-purification v1.jpg

Bielefeld 2011 Bead-Coating v1.jpg

IGEM Bielefeld Project.jpg


Data For Our Favorite New Parts

  1. [http://partsregistry.org/Part:BBa_K525305 Main Page] - Fusion Protein of S-Layer SgsE and mCitrine: This fluorescent S-layer fusion protein is used to characterize purification methods and to demonstrate the S-layer's ability to self-assemble on surfaces.
  2. [http://partsregistry.org/Part:BBa_K525515 Main Page] - Fusion Protein of BisdA and BisdB: This fusion protein improves the bisphenol A degradation in E. coli compared to the so far in the partsregistry existing BPA degrading BioBricks.
  3. [http://partsregistry.org/Part:BBa_K525710 Main Page] - NAD+-dependent DNA ligase from E. coli : This enzyme enables determination of NAD+ even in very low concentrations by coupling it with a molecular beacon based assay.


Data For Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K123000:Experience Experience] - BisdA degrades Bisphenol A when used with BisdB, BBa_K123000 (University of Alberta, iGEM 2008): Complete degradation of 120 mg L-1 Bisphenol A with polycistronic bisdAB gene in 30-33 h. Even faster (21-24 h) when using a fusion protein of BisdA and BisdB.
  2. [http://partsregistry.org/Part:BBa_K123001:Experience Experience] - BisdB degrades Bisphenol A when used with BisdA, BBa_K123001 (University of Alberta, iGEM 2008): Complete degradation of 120 mg L-1 Bisphenol A with polycistronic bisdAB gene in 30-33 h. Even faster (21-24 h) when using a fusion protein of BisdA and BisdB.


We've Also Characterized the Following Parts

  1. [http://partsregistry.org/Part:BBa_K525405 Main Page] - Fusion Protein of S-Layer SbpA and mCitrine: This fluorescent S-layer fusion protein is used to characterize purification methods and to demonstrate the S-layer's ability to self-assemble on surfaces.
  2. [http://partsregistry.org/Part:BBa_K525512 Main Page] - Polycistronic expression of BisdA and BisdB: This is the version of BPA degrading BioBricks found in the partsregistry - comparison to our fusion protein <partinfo>K525515</partinfo>.