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| | ==Sunday== | | ==Sunday== |
| - | Lab work done by: Alyssa Henning & Bill Jo
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| - | *Successfully miniprepped 1 sample of GFP + avitag (the second sample got messed up)
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| - | *Ran a gel on the RFP that was PCRed on Saturday. We also cut out the bands.
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| - | *Sean will innoculate 6 tubes of GFP-containing bacteria--1 tube for us, and 5 tubes for CURIE.
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| | | | |
| | ==Monday== | | ==Monday== |
| - | Lab work done by: Charlie Chung & Youjin Cho
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| - | *Gel purified 2 RFP PCR samples from Friday.
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| - | *Miniprepped 2 samples of GFP + avitag.
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| | | | |
| | ==Tuesday== | | ==Tuesday== |
| - | Lab Work Done By: James Mathew
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| - | Submitted GFP+avitag genes for synthesis
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| - |
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| - | Submitted pZE-12 backbone for subcloning of light sensor
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| | | | |
| | ==Wednesday== | | ==Wednesday== |
| - | Lab Work Done By: James Mathew
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| - |
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| - | Set up ligation reaction of pZE-12 backbone with the primer dimer insert.
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| - | Digestion of Backbone
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| - | - 26 ul Backbone plasmid = 2 ug DNA
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| - | - 5 ul Buffer #4 (optimal for SphI-HF and ClaI-HF)
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| - | - 1 ul SphI & 1 ul ClaI
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| - | - 16.5 ul H20
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| - | left in water bath for one hour
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| - | - 1 ul CIAP added to digestion reaction
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| - | left in water bath for 30 minutes
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| - |
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| - | Ran Digestion Product through gel for 30 minutes at 120 V.
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| - | Lane 1: 2 ul 1kb Ladder + 2 ul 6X Dye + 8 ul H20
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| - | Lane 2: 25 ul digestion reaction + 5 ul 6X Dye
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| - | Lane 3: 25 ul digestion reaction + 5 ul 6X Dye
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| - |
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| - | Gel Purification using Qiagen Kit
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| - |
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| - | NanoDrop of Samples
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| - | Concentration:
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| - | Label:
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| - |
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| - | Ligation Reaction
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| - | Label:
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| - |
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| - | Ligation done overnight for transformation on 7/21/11
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| | | | |
| | ==Thursday== | | ==Thursday== |
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