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Team:Paris Liliane Bettencourt/Notebook/2011/08/08/
From 2011.igem.org
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== Cyrille == | == Cyrille == | ||
Latest revision as of 16:08, 27 August 2011
Contents |
Cyrille
Danyel & Camille
T7 amber
8 clones were picked from the no.2 plate of 200µL and incubated in LB at 37ºC.
Kevin
PCR Phusion again
Gradient for 49°C to 68°C for Dave Lane plasmids.
Protocol for each tube :
- qsp H2O 50uL (32uL)
- 10X Buffer GC 10uL
- Primers FW and RV 2,5uL
- Template DNA 1ug
- DMSO 1,5uL
- Polymerase 0,5uL
