Team:Paris Liliane Bettencourt/Notebook/2011/08/08/

From 2011.igem.org

Team IGEM Paris 2011

Contents

Cyrille

Danyel & Camille

T7 amber

8 clones were picked from the no.2 plate of 200µL and incubated in LB at 37ºC.

Kevin

PCR Phusion again

Gradient for 49°C to 68°C for Dave Lane plasmids.

Protocol for each tube :

  • qsp H2O 50uL (32uL)
  • 10X Buffer GC 10uL
  • Primers FW and RV 2,5uL
  • Template DNA 1ug
  • DMSO 1,5uL
  • Polymerase 0,5uL