User:Allancrossman

From 2011.igem.org

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Allan Crossman, member of [[Team:Edinburgh|Edinburgh 2011]].
Allan Crossman, member of [[Team:Edinburgh|Edinburgh 2011]].
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==Personal TODO list==
 
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* Work out complete sequence of the PlacLacZ-INP we have and prepare for Gibson using it.
 
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* RFC for BioSandwich
 
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* Table of all our stuff, whether it has RBS, start codon, stop codon, etc.
 
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* Add hints.
 
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* Plan for pIII beads reactor?
 
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* Can we suppress the hover-over thing for pages with no hard words?
 
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* [[Team:Edinburgh/Carotenoids]]
 
==Notes to self==
==Notes to self==
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* [[Judging]]
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* [https://igem.org/Results?year=2011 2011 full results]
* [http://mic.sgmjournals.org/content/142/7/1659.full.pdf+html bglX info]
* [http://mic.sgmjournals.org/content/142/7/1659.full.pdf+html bglX info]
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* We must have a "[https://igem.org/Sample_Data_Page data page]" that explains the system and links to actual data which '''must be on the Registry'''.
 
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* We must have (somewhere) a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.
 
* [[User:Allancrossman/Gibson]] and [[User:Allancrossman/Gibson2]]
* [[User:Allancrossman/Gibson]] and [[User:Allancrossman/Gibson2]]
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* ''inaZ'' is [http://www.ncbi.nlm.nih.gov/nuccore/71553748?from=1850733&to=1855854 here], broken by an IS.
 
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* [http://www.lifesci.dundee.ac.uk/groups/tracy_palmer/docs/signals.htm Substrates of TAT pathway in ''E. coli'']
 
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* [https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS strengths from Warsaw 2010]
 
* [http://www.ncbi.nlm.nih.gov/nuccore/AF130864.1 pG8SAET]
* [http://www.ncbi.nlm.nih.gov/nuccore/AF130864.1 pG8SAET]
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* [[Special:Watchlist]]
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==Large display of small GIF==
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==Stuff others are doing==
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<html>
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<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:622px;"><a href="/File:Syn-Variants.png" class="image"><img alt="" src="/wiki/images/d/df/Syn.gif" width="620" height="310" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/File:Syn.gif" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Non-synergistic versus synergistic systems. Dark regions are places where the cellulose has been degraded down to free glucose molecules.</div></div></div></div>
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</html>
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==Video game concept==
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You control a humble ribosome. You are powered by sugar. Outside the cell is cellobiose floating around. You can express B-glucosidase on the cell exterior to get more sugar. You can express other stuff. There's cellulose floating around too. You can express cellulases. There's phage. You can express proteases or somesuch?
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==Lignin degradation==
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* Requires glucose oxidase to supply H2O2, a cofactor for other enzymes...
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** Lignin peroxidase
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** Manganaese peroxidase
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** Laccase?
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Some of these use flavin cofactors but it seems proteins using these can be successfully displayed using INP (Van Bloois ''et al'', 2009).
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==Gold medal criteria==
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* [https://2011.igem.org/Special:Contributions/Yazbo91 Contributions: Yassen]
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* Our wiki watch page is clearly a type of collaboration.
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* [https://2011.igem.org/Special:Contributions/L.Kopec Contributions: Lukasz]
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* Cooperation with Trieste re: ''C. fimi'' BGlu sequence.
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* [https://2011.igem.org/Special:Contributions/mclee Contributions: Lee]
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* Cooperation with Trieste re: ''C. fimi'' BGlu assay.
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* [https://2011.igem.org/Special:Contributions/sylvia.ispasani Contributions: Sylvia]
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* Registry update for K415151.
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* [https://2011.igem.org/Special:Contributions/Fionntom Contributions: Fionn]
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* Registry update for K392008.
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* [https://2011.igem.org/Special:Contributions/Di.L Contributions: Di]
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* Registry update for K265008.
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* All our human practices stuff.
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==References==
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==Competitors in our track==
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* Van Bloois E, Winter RT, Janssen DB, Fraaije MW (2009) [http://www.springerlink.com/content/d471504054865565/fulltext.pdf Export of functional ''Streptomyces coelicolor'' alditol oxidase to the periplasm or cell surface of ''Escherichia coli'' and its application in whole-cell biocatalysis]. ''Applied Microbiology and Biotechnology'' '''83''': 679-687 (doi: 10.1007/s00253-009-1904-0).
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: '''Progress to MIT:'''
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:: [[Team:Edinburgh]] (gold)
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:: [[Team:Johns_Hopkins]] (bronze)
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:: [[Team:UTP-Panama]] (bronze)
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:: [[Team:Washington]] (gold, Americas overall winner)
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:: [[Team:Yale]] (gold, Americas finalist)
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: '''Did not progress:'''
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:: [[Team:Alberta]]
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:: [[Team:Debrecen_Hungary]]
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:: [[Team:HIT-Harbin]]
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:: [[Team:Korea_U_Seoul]]
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:: [[Team:Nevada]]
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:: [[Team:Tec-Monterrey]]
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:: [[Team:Tianjin]]
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:: [[Team:UNAM-Genomics_Mexico]]
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:: [[Team:UST-Beijing]]
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:: [[Team:WashU]]
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Latest revision as of 18:38, 28 October 2011