Team:Lyon-INSA-ENS/Realisation/Notebook/Week1

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<FONT COLOR="green"> <b>PCR and mutagenesis of rcn, csgBA, csgBAEFG</b> </FONT> <br><br>
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<FONT COLOR="orange"> <b> Extraction of NiCoT </b></FONT> <br><br>
Culture of NM 522 cells for later transformations
Culture of NM 522 cells for later transformations
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<FONT COLOR="green"> <b>PCR and mutagenesis of rcn, csgBA, csgBAEFG</b> </FONT> <br><br>
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<FONT COLOR="orange"> <b> Extraction of NiCoT </b></FONT> <br><br>
<b>Extraction</b> of plasmid with NicoT gene from filter paper. <b>Transformation</b> of the extracted DNA in NM522 strain using TSS. Culture and selection of transformants on ampicilline plate during the week end.
<b>Extraction</b> of plasmid with NicoT gene from filter paper. <b>Transformation</b> of the extracted DNA in NM522 strain using TSS. Culture and selection of transformants on ampicilline plate during the week end.
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Latest revision as of 23:34, 21 September 2011







Week 1


From Thursday the 16th of June to Friday the 17th of June 2011





Thursday


Extraction of NiCoT

Culture of NM 522 cells for later transformations





Friday


Extraction of NiCoT

Extraction of plasmid with NicoT gene from filter paper. Transformation of the extracted DNA in NM522 strain using TSS. Culture and selection of transformants on ampicilline plate during the week end.







Previous Week Next Week








ENS assystem Biomérieux INSA INSA