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Team:Valencia/Notebook/Week11
From 2011.igem.org
WEEK 11
Engineering: The apparatus is almost finished. We are now concentrated on the decoration of the artifact
pH-stat: The evolution of the six cultures this week was not what we expected either, although culture number 6 with analytical water and without reflector survived better. We suspected that it was not performing as expected because of the concentration of fertilizer, so by the end of the week, seeing that they were few cells left , instead of rejecting them, we added 2 ml of fertilizer per flask to see if that could provoke an increase by the following Monday.
Colicin production: First, we grow the E. coli carrying the bacteriocins (col G et H). We also perform a viable count for each strain, to see their productivity. Now we start thinking of ways to quantify or purify the bacteriocins. We also perform a plasmid extraction on both strains. We receive another bacteriocin! (microcin C51). Thanks to Inna! We note that, ended the summer holidays, we are receiving almost everything we needed. The problem is we are running short of time!
pH-stat: The evolution of the six cultures this week was not what we expected either, although culture number 6 with analytical water and without reflector survived better. We suspected that it was not performing as expected because of the concentration of fertilizer, so by the end of the week, seeing that they were few cells left , instead of rejecting them, we added 2 ml of fertilizer per flask to see if that could provoke an increase by the following Monday.
Colicin production: First, we grow the E. coli carrying the bacteriocins (col G et H). We also perform a viable count for each strain, to see their productivity. Now we start thinking of ways to quantify or purify the bacteriocins. We also perform a plasmid extraction on both strains. We receive another bacteriocin! (microcin C51). Thanks to Inna! We note that, ended the summer holidays, we are receiving almost everything we needed. The problem is we are running short of time!
