Team:Paris Liliane Bettencourt/Notebook/2011/08/27/

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Danyel

pT7+RBS+GFP+Ter

Results:

Camille

Transformation seemed to work. To verify the construct was really in my strains I decided to do a PCR colony for five clones. I used for that the primers VF2 and VR. I also digested minipreps from Danyel of the strains containing the tRNA one strain with a terminator behind the second one without. In order to clon the pHyperspank before the tRNA. I did a gel, extracted the bands. I obtained good [DNA], 12.8ng/µL fr the tRNA + terminator strain and 17.9ng/µL for the other one.

I launched the ligation overnight. The PCR colony resulted in the selection of two over the five clones that looked good. I launched them for an overnight liquid culture.