Team:Paris Liliane Bettencourt/Notebook/2011/08/22/

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Kevin

Results of PCR Colony

Gel of PCR Colony - Column 1.2.1 - 1.2.2 - 1.3.1 - 1.3.2 - 2.2.1 - 2.2.2 - 2.3.1 - 2.3.2 - 3.7.1 - 3.7.2 - 3.8.1 - 3.8.2 - 4.7.1 - 4.7.2 - 4.8.1 - 4.8.2

So it seems that 3 clones of tetR:YFP (2.2.1 - 2.2.2 - 2.3.1) and 2 clones of tetO array (4.7.1 -4.8.2) works.
Sent it today to sequencing.

Going to vacation, all instructions are in a paper in the computer room if someone wants to progress in this project !

Axel

ligation of ComS biobricked plasmids

ligation with PSB1C3 (digested in Ecor1 and Pst1) / ComS digested in Ecor1 and Pst1 ligation with K143053 (digested in Spe1 and Pst1) / ComS digested in Xba1 and Pst1

after transformation: get a lot of colonies. Sent to sequencing: it seems that the expected ComS is not ComS

Camille

As we did for the tRNA we decided to synthesize the promoter Hyperspank ba y annealing extension with Klenow polymerase. That polymerase is known for elongating primers at 37°C that way we prevent the primers to detach when the product is heated to reach the elongation temperature of the Taq or phusion. I launched one control and three tubes.