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• Team:Potsdam Bioware/Labjournal/August part 2
  ...er contains a complete myc tag for insertion of myc into the phage display vector * this may lead to the low yield of geneIII target DNA after PCR using entire vector pak bla KDIR as template
  134 KB (19,391 words) - 13:03, 21 September 2011
• Team:Potsdam Bioware/Labjournal/September part 1
  ...ackground-color: rgb(254, 122, 122); font-weight: bold;">Digest of pUP089 (vector) </h3> <b>Aim:</b> generate vector for ligation and transformation to generate a libary
  151 KB (21,501 words) - 17:06, 21 September 2011
• Team:TU Munich/lab/notebook/part3
  ...st:</p><p>pSB3C5: S and P cut by Alex on 23.08.11, CAM resistance, 2,7 kbp vector backbone</p><p>PCR product of BBa_K322127 with primers BBa_K568000, derived ...he following bands were expected: 3.1 kbp for lacZ with rbs and 2 kbp for vector pSB1A2. 3.4 kbp for T7prom in pSB1AK8. The bands are excised from the gel a
  121 KB (20,136 words) - 01:54, 22 September 2011
• Team:Edinburgh/Lab Notebook
  ...e (so the final thing becomes E-X-B-Plac-lacZ&alpha;-S-P). This gives us a vector that we like. For the principles behind the use of lacZ&alpha; see [http:// ...y DNA sequence can easily have the sites needed for incorporation into the vector added (by PCR) at both ends, whereas in the standard RFC10 system the two m
  69 KB (11,148 words) - 15:33, 3 October 2011
• Team:Potsdam Bioware/Labjournal/September part 2
  ...yle="background-color: rgb(254, 122, 122); font-weight: bold;">miniprep of pSB1C3+mdnABCDE with and without T7 promotor</h3> <b>Aim:</b> generate BioBrick of mdnABCDE, amplify mdnABCDE for ligation in pSB1C3<br>
  58 KB (8,180 words) - 00:26, 22 September 2011
• Team:TU Munich/lab/notebook/part4
  ...ee samples of pSB6A1 with I746907, different I:V-ratios; pSB6A1 E,P cut as vector background) was purified today using glycogen/ethanol precipitation accordi Plate 4 (vector background) spread with concentrated bacteria solution showed 85 colonies.
  78 KB (13,436 words) - 22:42, 28 October 2011
• Team:Potsdam Bioware/Labjournal/August part 1
  <b>Further tasks:</b><br> ligation into vector * 6 µl (ca 70 ng) digested vector pARW089 (NarI, AatII)
  117 KB (17,234 words) - 01:03, 22 September 2011
• Team:Uppsala-Sweden/Notebook
  pSB1C3-J04450 (CmR backbone) – Lei & Sibel <br> pSB3T5-J04450 (low copy vector tet, ori P15A) – From Erik G <br>
  55 KB (8,809 words) - 15:16, 25 October 2011
• Team:KIT-Kyoto/Notebook/LabNote/GFPMLF9
  ...ickgelextractionkit.aspx|QIAquick Gel Extraction Kit] and extracted DNA of pSB1C3 from the gel. ...ickgelextractionkit.aspx|QIAquick Gel Extraction Kit] and extracted DNA of pSB1C3 from the gel.
  37 KB (6,399 words) - 03:45, 6 October 2011
• Team:NTNU Trondheim/Journal
  *6:1 molar ratio of insert to vector (~10ng vector) *Add (8.5 - vector and insert volume)μl ddH2O
  64 KB (10,273 words) - 15:51, 21 September 2011
• Team:Nevada/Notebook/Weeks912
  ...ata confirms the TRC promoter was successfully cloned into the TOPO2.1 PCR vector! ...I and checked on 0.7% gel. The results for this gel were good. ThiE/Topo Vector was digest with EcoRI and PstI and checked on a 0.7% gel. The gel was not
  30 KB (5,035 words) - 03:20, 29 September 2011
• Team:Potsdam Bioware/Labjournal/June
  * Sequence of vector pARW071, concentration 857.3 ng/ µl * Sequence of vector pARW089, concentration 626.7 ng/ µl
  70 KB (9,678 words) - 01:31, 22 September 2011
• Team:UNICAMP-EMSE Brazil/Notebook/17 September 2011
  :# Quantification of linearized pSB1C3 vector purified from the gel (volume of ladder applied 6 uL; sample: 5 uL DNA +1 u ! scope="col" style="background:#efefef;" | Total size (gene+vector)
  14 KB (2,168 words) - 14:09, 19 September 2011
• Team:UT-Tokyo/LabNote
  | || || || pSB1C3 || class="number" | 831 | pSB1C3 || || Linearized plasmid backbone || pSB1C3 || class="number" | 2070
  186 KB (30,010 words) - 20:25, 5 October 2011
• Team:Nevada/Notebook/Weeks1316
  ...in the Topo vector (pUC57) were grown, and minipreped. The BTE samples and pSB1C3 were digested with Xba I and Pst I and ligated. Ligation was transformed u BTE with sigma 70 promoter and pSB1C3 were digested with EcoRI and PstI and ligated, transformed, and cultured.<b
  25 KB (4,235 words) - 03:58, 29 September 2011
• Team:Lethbridge/Notebook/Lab Work/Group1
  <i>Transformation of K325909 in pSB1C3 and K325229 in pSB1C3 from 2011 Kit Plate</i> * 10µL of Milli-Q water and extraction of K325909 in pSB1C3 from the 2011 kit plate (17m) were mixed together.
  22 KB (3,366 words) - 03:32, 29 September 2011
• Team:Potsdam Bioware/Labjournal/October
  * Ligation with pSB1C3 * Ligation with pSB1C3
  89 KB (13,149 words) - 18:03, 28 October 2011
• Team:UNIPV-Pavia/Freezer
  <td class="row"><b>Vector</b></td> <td class="row">Low copy BioBrick standard vector - chloramphenicol antibiotic resistance</td>
  31 KB (4,892 words) - 13:30, 9 November 2011
• Team:UPO-Sevilla/Project/Notebook/MiniTn7
  ... replicative in Enterobacteriaceae to maintain the mini-Tn7. I located the vector in the lab strain collection, set up an inoculum, purified the plasmid DNA, ...ab in Colorado State University. We will use this as a template to amplify vector sequences adding SfiI restriction sites. I had problems to get amplificatio
  35 KB (5,556 words) - 10:07, 28 October 2011
• Team:UNIPV-Pavia/Calendar/August/week4
  <td class="row"><b>Vector</b></td> <td class="row"><b>Vector volume (&mu;l)</b></td>
  18 KB (3,015 words) - 10:12, 18 September 2011