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  • Team:Potsdam Bioware/Labjournal/August part 2
    <b>Time:</b> 2011-08-18, 15:00-18:00 <br> <b>Time:</b> 2011-08-19, 14:00-14:15<br>
    134 KB (19,391 words) - 13:03, 21 September 2011
  • Team:Potsdam Bioware/Labjournal/August part 1
    * Total volume: 15 µl * Total volume: 200 µl (approx. 15 µl per sequencing reaction)
    117 KB (17,234 words) - 01:03, 22 September 2011
  • Team:Potsdam Bioware/Labjournal/June
    <b>Time:</b> 2011-06-01,15:30-21:00<br> | 94 || 15 || Denaturation
    70 KB (9,678 words) - 01:31, 22 September 2011
  • Team:WHU-China/calendarjs
    ...."+a[12])*1E3:0);if(a[14]){b=Number(a[16])*60+(a[18]?Number(a[18]):0);b*=a[15]=="-"?1:-1;e=new Date(+e+b*60*1E3)}}return e}function mb(a){if(typeof a=="n ...a+"-widget-header",O=Wa+"-widget-content",x,A,ta,za,Da,Ea=i("slotMinutes")%15==0;x="<table style='width:100%' class='fc-agenda-days fc-border-separate' c
    72 KB (13,661 words) - 13:53, 24 October 2011
  • Script
    ...,41r-32,-26r31,-34r-42,-15r15,-35r41,19r-5,-45r44,0r-6,46r41,-20r15,35r-44,15","w":148},"^":{"d":"82,-252r52,0r77,143r-64,0r-39,-81r-40,81r-64,0","w":214
    34 KB (6,310 words) - 02:45, 7 September 2011
  • Team:WHU-China/Notebook/Daytoday
    <span style="font-weight:bolder">4/14~4/15</span>, preparing materials: such as DH5α, pDsRed -Express Vector, amp, ka <span style="font-weight:bolder">6/2~6/15</span>, produce the competent cell, use T4 ligase to join DNA fragments, an
    29 KB (3,834 words) - 14:34, 26 October 2011
  • Team:Potsdam Bioware/Labjournal/September part 2
    |Denaturation|| 94°C|| 15 s |DenaturationII || 94°C|| 15 s
    58 KB (8,180 words) - 00:26, 22 September 2011
  • Team:Potsdam Bioware/Labjournal/September part 1
    | 15 || pPDV089, clone 4 || 12|| ca. 1400 ** mycN: 15.2 ng/µl
    151 KB (21,501 words) - 17:06, 21 September 2011
  • Team:Potsdam Bioware/Labjournal/July
    <b>Time:</b> 2011-06-30,14:00-15:30<br> <b>Time:</b> 2011-06-24, 15:30-16:00 <br>
    55 KB (8,148 words) - 21:15, 18 September 2011
  • Team:TU Munich/lab/notebook/part3
    Annealing: 60°C, 15 s ...ng Zymo DNA Clean +Concentrator 25</p><p><i>ligation:</i></p><p>sample 1: 15µl PCR product 5µl vector
    121 KB (20,136 words) - 01:54, 22 September 2011
  • Team:Minnesota/Notebook
    | Thu, 07 Apr 2011 15:14:00 GMT | Mon, 18 Jul 2011 15:28:28 GMT
    32 KB (3,783 words) - 22:46, 27 September 2011
  • Team:Penn/project/protocols/
    15 mL conical tube<br /> <p>1. Warm media, PBS, trypsin in water bath to 37 C (Takes approximately 10-15 minutes)<br />
    86 KB (13,981 words) - 04:46, 29 September 2011
  • Team:Johns Hopkins/Notebook/Vio
    Followed same protocol as 6/24, trying not to pick too many cells, using .15µl of the 5µM primer mix, and extending the initial denaturation to 10 min =====7/15/2011=====
    78 KB (11,015 words) - 22:10, 28 October 2011
  • Team:Potsdam Bioware/Labjournal/October
    * centrifuge cell culture at 5000 x g/ 15 min * fill supernatant in a new 50 ml falcon and centrifuge again (5000 g/15 min)
    89 KB (13,149 words) - 18:03, 28 October 2011
  • Team:UT-Tokyo/Data/Method
    :#Centrifuge the incubative tube at 3,000g for 15 min with soft brake. :#Incubation at 16 &deg;C for 15-30 min.
    15 KB (2,260 words) - 23:25, 5 October 2011
  • Team:Michigan/Notebook
    ==Week 2 (5/15/2011-5/21/2011)== '''6/15/2011'''
    73 KB (11,794 words) - 05:23, 29 September 2011
  • Team:UT-Tokyo/LabNote
    ==='11/06/15(Wed)=== ==='11/07/15(Fri)===
    186 KB (30,010 words) - 20:25, 5 October 2011
  • Team:TU Munich/lab/notebook/part4
    </li><li>Ligation 15 </li><li>Ligation 15
    78 KB (13,436 words) - 22:42, 28 October 2011
  • Team:Debrecen Hungary/Protocols
    15, 50 ml tubes 1. Prepare the laminar flow box: turn on the ventillation, wait for 15 minutes, clean the bottom and the glass of the hood with 70% alcohol, and w
    51 KB (7,937 words) - 05:49, 22 September 2011
  • Team:Bielefeld-Germany/Backup-Protocols
    * Heat-shock cells for 15–20 seconds in a water bath at exactly 42 °C. Do not shake. ...Germany/Protocols#Used_enzymes | SAP (shrimp alcaline phosphatase)]] and 1.15 µL 10x SAP buffer to destination plasmid mix, continue incubation at 37 °
    62 KB (8,860 words) - 12:04, 15 September 2011

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